Isolation of fungi from Lake Vostok accretion ice

被引：38

作者：

D'Elia, Tom ^{[1
]}

Veerapaneni, Ram ^{[1
]}

Theraisnathan, Vincent ^{[1
]}

Rogers, Scott O. ^{[1
]}

机构：

[1] Bowling Green State Univ, Dept Biol Sci, Bowling Green, OH 43403 USA

来源：

MYCOLOGIA | 2009年 / 101卷 / 06期

关键词：

Antarctica; heterotrophs; ITS; subglacial lake; MICROORGANISMS; ANTARCTICA; DIVERSITY; DISCOVERY; GROWTH; SOILS; CORE; FATE;

D O I：

10.3852/08-184

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Here we report the characterization of fungi from 10 accretion ice sections (3300-5100 y old) as well as two deep glacial ice sections that are close to the bottom of the glacier (1 000 000-2 000 000 y old) from the Vostok, Antarctica, 5G ice core. Fungi were characterized by fluorescence microscopy Culturing and sequence analyses of ribosomal DNA internal transcribed spacers. A total of 270 fungal colonies were cultured from the accretion ice of subglacial Lake Vostok and an additional 14 from the glacial ice immediately above the accretion ice. Mean concentrations were 0-4.42 cells mL(-1) ice meltwater of which 0-100% exhibited viability (as determined by fluorescence microscopy). Thirty-one unique fungal ribosomal DNA sequences (28 from accretion ice and three from glacial ice) were determined and compared to recent taxa. The results, plus tests for growth at low temperatures, indicated that Lake Vostok contains a mixture of heterotrophic psychrotolerant fungal species. This indicates that the lake is not sterile but contains a unique ecosystem.

引用

页码：751 / 763

页数：13

共 48 条

[1] Use of different methods for discovery of ice-entrapped microorganisms in ancient layers of the Antarctic glacier [J].

Abyzov, SS ;

Hoover, RB ;

Imura, S ;

Mitskevich, IN ;

Naganuma, T ;

Poglazova, MN ;

Ivanov, MV .

SPACE LIFE SCIENCES: SEARCH FOR SIGNATURES OF LIFE, AND SPACE FLIGHT ENVIRONMENTAL EFFECTS ON THE NERVOUS SYSTEM, 2004, 33 (08) :1222-1230

[2] Fungal diversity in soils and historic wood from the Ross Sea Region of Antarctica [J].

Arenz, Brett E. ;

Held, Benjamin W. ;

Jurgens, Joel A. ;

Farrell, Roberta L. ;

Blanchette, Robert A. .

SOIL BIOLOGY & BIOCHEMISTRY, 2006, 38 (10) :3057-3064

[3]

Bell R., 2005, Life in Ancient Ice, P251

[4] Origin and fate of Lake Vostok water frozen to the base of the East Antarctic ice sheet [J].

Bell, RE ;

Studinger, M ;

Tikku, AA ;

Clarke, GKC ;

Gutner, MM ;

Meertens, C .

NATURE, 2002, 416 (6878) :307-310

[5] Psychrooligotrophic fungi from Arctic soils of Franz Joseph Land [J].

Bergero, R ;

Girlanda, M ;

Varese, GC ;

Intili, D ;

Luppi, AM .

POLAR BIOLOGY, 1999, 21 (06) :361-368

[6]

Bulat S.A., 2004, Int. J. Astobiol, V1, P1, DOI [10.1017/S1473550404001879, DOI 10.1017/S1473550404001879]

[7] Yeasts in high Arctic glaciers: the discovery of a new habitat for eukaryotic microorganisms [J].

Butinar, Lorena ;

Spencer-Martins, Isabel ;

Gunde-Cimerman, Nina .

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 2007, 91 (03) :277-289

[8] Limnological conditions in Subglacial Lake Vostok, Antarctica [J].

Christner, Brent C. ;

Royston-Bishop, George ;

Foreman, Christine M. ;

Arnold, Brianna R. ;

Tranter, Martyn ;

Welch, Kathleen A. ;

Lyons, W. Berry ;

Tsapin, Alexandre I. ;

Studinger, Michael ;

Priscu, John C. .

LIMNOLOGY AND OCEANOGRAPHY, 2006, 51 (06) :2485-2501

[9] Isolation of microbes from Lake Vostok accretion ice [J].

D'Elia, Tom ;

Veerapaneni, Ram ;

Rogers, Scott O. .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (15) :4962-4965

[10] Contributions of an ancient evaporitic-type reservoir to subglacial Lake Vostok chemistry [J].

De Angelis, M ;

Petit, JR ;

Savarino, J ;

Souchez, R ;

Thiemens, MH .

EARTH AND PLANETARY SCIENCE LETTERS, 2004, 222 (3-4) :751-765

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