How does radiation damage in protein crystals depend on x-ray dose?

被引：95
|
作者：
Sliz, P
Harrison, SC [1 ]
Rosenbaum, G
机构：
[1] Harvard Univ, Sch Med, Howard Hughes Med Inst, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA
[2] Univ Georgia, Dept Biochem, SER CAT, APS, Argonne, IL 60439 USA
来源：
STRUCTURE | 2003年 / 11卷 / 01期
关键词：
protein crystals; radiation damage; synchrotron radiation; X-ray crystallography;
D O I：
10.1016/S0969-2126(02)00910-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Is radiation damage to cryopreserved protein crystals strictly proportional to accumulated dose at the high-flux density of beams from undulators at third-generation synchrotron sources? The answer is "yes," for overall damage to several different kinds of protein crystals at flux densities up to 10(15) ph/sec/mm(2) (APS beamline 19-ID). We find that, at 12 keV (1 Angstrom wavelength), about ten absorbed photons are sufficient to "kill" a unit cell. As this corresponds to about one elastically scattered photon, each unit cell can contribute only about one photon to total Bragg diffraction. The smallest crystal that can yield a full data set to 3.5 Angstrom resolution has a diameter of about 20 mum (100 Angstrom unit cell).
引用
收藏
页码：13 / 19
页数：7
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