DspE of Erwinia amylovora interacts with receptor kinases of apple

被引：1

作者：

Meng, X ^{[1
]}

Bonasera, JM ^{[1
]}

Kim, JF ^{[1
]}

Nissinen, RM ^{[1
]}

Beer, SV ^{[1
]}

机构：

[1] Cornell Univ, Dept Plant Pathol, Ithaca, NY 14853 USA

来源：

PROCEEDINGS OF THE IXTH INTERNATIONAL WORKSHOP ON FIRE BLIGHT | 2002年 / 590期

关键词：

pathogenicity; yeast-two-hybrid; virulence gene; fire blight;

D O I：

10.17660/ActaHortic.2002.590.71

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

Erwinia amylovora causes fire blight of apple, pear, and other rosaceous plants and elicits plant defense responses in nonhost plants. Previous research indicated that a disease specific gene, dspE, from E. amylovora is essential for pathogenicity. DspE is a homolog of AvrE of Pseudomonas syringae. The LexA yeast-two-hybrid system was used to seek DspE-interacting proteins from apple to further explore its role in pathogenicity. Bait constructs consisting of several portions of the dspE gene were made in the expression vector pGlida. A representative cDNA library of apple (Malus x domestica cv. 'Royal Gala') was constructed in the vector pB42AD. 198 putative interacting clones were selected and re-screened to yield four unique, positive clones. A Blast search of cDNA sequences of the four clones indicated that all had protein kinase domains and belonged to LRR receptor-like kinases. The full-length sequences of the four clones were identified. Further characterization of the cDNAs and the encoded kinases are underway. Studies of the gene function are in progress.

引用

页码：463 / 466

页数：4

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