Determination of Helicobacter pylori virulence by analysis of the cag pathogenicity island isolated from Iranian patients

被引：29
作者：
Baghaei, K. [3 ]
Shokrzadeh, L. [3 ]
Jafari, F. [3 ]
Dabiri, H. [3 ]
Yamaoka, Y. [1 ,2 ]
Bolfion, M. [3 ]
Zojaji, H. [3 ]
Aslani, M. M. [3 ,4 ]
Zali, M. R. [3 ]
机构：
[1] Michael E DeBakey Vet Affairs Med Ctr, Dept Med Gastroenterol, Houston, TX 77030 USA
[2] Baylor Coll Med, Houston, TX 77030 USA
[3] Shaheed Beheshti Univ, Res Ctr Gastroenterol & Liver Dis, Tehran, Iran
[4] Inst Pasteur Iran, Dept Microbiol, Enterobacteriarae Lab, Tehran, Iran
来源：
DIGESTIVE AND LIVER DISEASE | 2009年 / 41卷 / 09期
基金：
美国国家卫生研究院;
关键词：
cag pathogenicity island; Gastroduodenal diseases; Helicobacter pylori; Iran; GASTRIC EPITHELIAL-CELLS; IV SECRETION; CLINICAL-RELEVANCE; GENE POLYMORPHISMS; VACA GENOTYPES; DISEASE; STRAINS; INFECTION; RISK; TRANSLOCATION;
D O I：
10.1016/j.dld.2009.01.010
中图分类号：
R57 [消化系及腹部疾病];
学科分类号：
摘要：
Background: The cog pathogenicity island (PAI), which can be divided into two parts, cagI and cagII, is the most well-known virulence factor of Helicobacter pylori, Aims: We investigated the association between genetic variations within the cog PAI (cagA and cagE in the cagI and cagT in the cagII) and clinical outcomes in Iranian patients. Subjects: A total of 231 patients including 182 patients with gastritis, 41 with peptic ulcer and 8 with gastric cancer. Methods: The presence of the cagA, cagE and cagT genes were measured by polymerase chain reaction and the results were compared with clinical outcomes and gastric histology. Results: The cagA, cagE and cagT genes were found in 154 (66.7%). 90 (39.0%) and 70 (30.3%) of clinical isolates. At least 144 (62.3%) strains possessed partially deleted cog PAI (e.g., 69 [29.9%] strains were cagA-positive, but cagE and cagT-negative). Conclusion: The single genes as well as the combination of genes in the cog PAI appeared not to be useful markers to predict H. pylori-related diseases in Iranian patients. The genomic sequences of the cog PAI in Iranian strains might be considerably different from those in other geographic locations. (C) 2009 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.
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页码：634 / 638
页数：5
相关论文
共 35 条
[1]   Helicobacter pylori CagA protein can be tyrosine phosphorylated in gastric epithelial cells [J].
Asahi, M ;
Azuma, T ;
Ito, S ;
Ito, Y ;
Suto, H ;
Nagai, Y ;
Tsubokawa, M ;
Tohyama, Y ;
Maeda, S ;
Omata, M ;
Suzuki, T ;
Sasakawa, C .
JOURNAL OF EXPERIMENTAL MEDICINE, 2000, 191 (04) :593-602
[2]   Translocation of the Helicobacter pylori CagA protein in gastric epithelial cells by a type IV secretion apparatus [J].
Backert, S ;
Ziska, E ;
Brinkmann, V ;
Zimny-Arndt, U ;
Fauconnier, A ;
Jungblut, PR ;
Naumann, M ;
Meyer, TF .
CELLULAR MICROBIOLOGY, 2000, 2 (02) :155-164
[3]   Role of type IV secretion in Helicobacter pylori pathogenesis [J].
Backert, Steffen ;
Selbach, Matthias .
CELLULAR MICROBIOLOGY, 2008, 10 (08) :1573-1581
[4]   Clinical relevance of Helicobacter pylori cagA and vacA gene polymorphisms [J].
Basso, Daniela ;
Zambon, Carlo-Federico ;
Letley, Darren P. ;
Stranges, Alessia ;
Marchet, Alberto ;
Rhead, Joanne L. ;
Schiavon, Stefania ;
Guariso, Graziella ;
Ceroti, Marco ;
Nitti, Donato ;
Rugge, Massimo ;
Plebani, Mario ;
Atherton, John C. .
GASTROENTEROLOGY, 2008, 135 (01) :91-99
[5]   Helicobacter pylori genetic diversity and risk of human disease [J].
Blaser, MJ ;
Berg, DE .
JOURNAL OF CLINICAL INVESTIGATION, 2001, 107 (07) :767-773
[6]  
BLASER MJ, 1995, CANCER RES, V55, P2111
[7]   cag, a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factors [J].
Censini, S ;
Lange, C ;
Xiang, ZY ;
Crabtree, JE ;
Ghiara, P ;
Borodovsky, M ;
Rappuoli, R ;
Covacci, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (25) :14648-14653
[8]   Classification and grading of gastritis - The updated Sydney System [J].
Dixon, MF ;
Genta, RM ;
Yardley, JH ;
Correa, P ;
Batts, KP ;
Dahms, BB ;
Filipe, MI ;
Haggitt, RC ;
Haot, J ;
Hui, PK ;
Lechago, J ;
Lewin, K ;
Offerhaus, JA ;
Price, AB ;
Riddell, RH ;
Sipponen, P ;
Solcia, E ;
Watanabe, H .
AMERICAN JOURNAL OF SURGICAL PATHOLOGY, 1996, 20 (10) :1161-1181
[9]  
Farshad S, 2007, SAUDI MED J, V28, P529
[10]   The significance of cagA and vacA subtypes of Helicobacter pylori in the pathogenesis of inflammation and peptic ulceration [J].
Gunn, MC ;
Stephens, JC ;
Stewart, JAD ;
Rathbone, BJ ;
West, KP .
JOURNAL OF CLINICAL PATHOLOGY, 1998, 51 (10) :761-764
1234