Lessons from peroxisome-deficient Chinese hamster ovary (CHO) cell mutants

被引：36

作者：

Fujiki, Yukio ^{[1
]}

Okumoto, Kanji ^{[1
]}

Kinoshita, Naohiko ^{[1
]}

Ghaedi, Kamran ^{[1
]}

机构：

[1] Kyushu Univ, Grad Sch, Fac Sci, Dept Biol,Higashi Ku, Fukuoka 8128581, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 2006年 / 1763卷 / 12期

关键词：

CHO cell mutant; genetic phenotype-complementation; peroxin; peroxisome ghost; Zellweger syndrome; patients' fibroblast; pathogenic gene;

D O I：

10.1016/j.bbamcr.2006.09.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cells with a genetic defect affecting a biological activity and/or a cell phenotype are generally called "cell mutants" and are a highly useful tool in genetic, biochemical, as well as cell biological research. To investigate peroxisome biogenesis and human peroxisome biogenesis disorders, more than a dozen complementation groups of Chinese hamster ovary (CHO) cell mutants defective in peroxisome assembly have been successfully isolated and established as a model system. Moreover, successful PEXgene cloning studies by taking advantage of rapid functional complementation assay of CHO cell mutants invaluably contributed to the accomplishment of isolation of pathogenic genes responsible for peroxisome biogenesis diseases. Molecular mechanisms of peroxisome assembly are currently investigated by making use of such mammalian cell mutants. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：1374 / 1381

页数：8

共 107 条

[1] A novel pex2 mutant:: catalase-deficient but temperature-sensitive PTS1 and PTS2 import [J].

Akiyama, N ;

Ghaedi, K ;

Fujiki, Y .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2002, 293 (05) :1523-1529

[2]

[Anonymous], NATURE ENCY HUMAN GE

[3]

[Anonymous], NATURE ENCY HUMAN GE

[4] DISORDERS OF PEROXISOME BIOGENESIS [J].

BRAVERMAN, N ;

DODT, G ;

GOULD, SJ ;

VALLE, D .

HUMAN MOLECULAR GENETICS, 1995, 4 :1791-1798

[5] Human PEX7 encodes the peroxisomal PTS2 receptor and is responsible for rhizomelic chondrodysplasia punctata [J].

Braverman, N ;

Steel, G ;

Obie, C ;

Moser, A ;

Moser, H ;

Gould, SJ ;

Valle, D .

NATURE GENETICS, 1997, 15 (04) :369-376

[6] An isoform of Pex5p, the human PTS1 receptor, is required for the import of PTS2 proteins into peroxisomes [J].

Braverman, N ;

Dodt, G ;

Gould, SJ ;

Valle, D .

HUMAN MOLECULAR GENETICS, 1998, 7 (08) :1195-1205

[7] Isolation of the human PEX12 gene, mutated in group 3 of the peroxisome biogenesis disorders [J].

Chang, CC ;

Lee, WH ;

Moser, H ;

Valle, D ;

Gould, SJ .

NATURE GENETICS, 1997, 15 (04) :385-388

[8] Pex5p, the peroxisomal cycling receptor, is a monomeric non-globular protein [J].

Costa-Rodrigues, J ;

Carvalho, AF ;

Fransen, M ;

Hambruch, E ;

Schliebs, W ;

Sá-Miranda, C ;

Azevedo, JE .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (26) :24404-24411

[9] PEX1 mutations in the Zellweger spectrum of the peroxisome biogenesis disorders [J].

Crane, DI ;

Maxwell, MA ;

Paton, BC .

HUMAN MUTATION, 2005, 26 (03) :167-175

[10] PEROXISOME-DEFICIENT MUTANTS OF HANSENULA-POLYMORPHA [J].

CREGG, JM ;

VANKLEI, IJ ;

SULTER, GJ ;

VEENHUIS, M ;

HARDER, W .

YEAST, 1990, 6 (02) :87-97

← 1 2 3 4 5 6 7 8 9 10 →