Exogenous glutathione alleviates chilling injury in postharvest bell pepper by modulating the ascorbate-glutathione (AsA-GSH) cycle

被引：181

作者：

Yao, Miaomiao ^{[1
]}

Ge, Wanying ^{[1
]}

Zhou, Qian ^{[1
]}

Zhou, Xin ^{[1
]}

Luo, Manli ^{[1
]}

Zhao, Yingbo ^{[1
]}

Wei, Baodong ^{[1
]}

Ji, Shujuan ^{[1
]}

机构：

[1] Shenyang Agr Univ, Coll Food Sci, 120 Dongling Rd, Shenyang 110866, Peoples R China

来源：

FOOD CHEMISTRY | 2021年 / 352卷

基金：

国家重点研发计划;

关键词：

Ascorbate-glutathione cycle; Capsicum annuum L; Glutathione; Low-temperature damage; Oxidative stress; OXIDATIVE-STRESS; SOLANUM-LYCOPERSICON; TOLERANCE; FRUIT; ACCUMULATION; ANTIOXIDANTS; RESISTANCE; REDUCTASE; DEFENSE; PATHWAY;

D O I：

10.1016/j.foodchem.2021.129458

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

We investigated the effect of exogenous glutathione (GSH) on chilling injury (CI) in postharvest bell pepper fruits stored at low temperature and explored the mechanism of this treatment from the perspective of the ascorbate-glutathione (AsA-GSH) cycle. Compared with the control, fruits treated with exogenous GSH before refrigeration displayed only slight CI symptoms and mitigated CI-induced cell damage after 10 d. Moreover, the treated peppers had lower lipid peroxidation product, H2O2, and O-2(-) content than those did the control. Glutathione treatment enhanced the ascorbate-glutathione cycle by upregulating CaAPX1, CaGR2, CaMDHAR1, and CaD-HAR1 and the antioxidant enzymes APX, GR, and MDHAR associated with the ascorbate-glutathione cycle. Glutathione treatment also increased ascorbate and glutathione concentrations. Taken together, our results showed that exogenous GSH treatment could alleviate CI in pepper fruits during cold storage by triggering the AsA-GSH cycle and improving antioxidant capacity.

引用

页数：8

共 40 条

[1] Glutathione reductase a unique enzyme: molecular cloning, expression and biochemical characterization from the stress adapted C4 plant, Pennisetum glaucum (L.) R. Br [J].

Achary, V. Mohan Murali ;

Reddy, Chinreddy S. ;

Pandey, Prachi ;

Islam, Tahmina ;

Kaul, Tanushri ;

Reddy, Malireddy K. .

MOLECULAR BIOLOGY REPORTS, 2015, 42 (05) :947-962

[2] Employing exogenous melatonin applying confers chilling tolerance in tomato fruits by upregulating ZAT2/6/12 giving rise to promoting endogenous polyamines, proline, and nitric oxide accumulation by triggering arginine pathway activity [J].

Aghdam, Morteza Soleimani ;

Luo, Zisheng ;

Jannatizadeh, Abbasali ;

Sheikh-Assadi, Morteza ;

Sharafi, Yavar ;

Farmani, Boukaga ;

Fard, Javad Rezapour ;

Razavi, Farhang .

FOOD CHEMISTRY, 2019, 275 :549-556

[3] Changes in red pepper antioxidants as affected by UV-C treatments and storage at chilling temperatures [J].

Andrade Cuvi, Maria J. ;

Vicente, Ariel R. ;

Concellon, Analia ;

Chaves, Alicia R. .

LWT-FOOD SCIENCE AND TECHNOLOGY, 2011, 44 (07) :1666-1671

[4] Determining the potential to breed for enhanced antioxidant status in Malus:: Mean inter- and intravarietal fruit vitamin C and glutathione contents at harvest and their evolution during storage [J].

Davey, MW ;

Keulemans, J .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2004, 52 (26) :8031-8038

[5] Get the Balance Right: ROS Homeostasis and Redox Signalling in Fruit [J].

Decros, Guillaume ;

Baldet, Pierre ;

Beauvoit, Bertrand ;

Stevens, Rebecca ;

Flandin, Amelie ;

Colombie, Sophie ;

Gibon, Yves ;

Petriacq, Pierre .

FRONTIERS IN PLANT SCIENCE, 2019, 10

[6] Salicylic-Acid-Induced Chilling- and Oxidative-Stress Tolerance in Relation to Gibberellin Homeostasis, C-Repeat/Dehydration-Responsive Element Binding Factor Pathway, and Antioxidant Enzyme Systems in Cold-Stored Tomato Fruit [J].

Ding, Yang ;

Zhao, Jinhong ;

Nie, Ying ;

Fan, Bei ;

Wu, Shujuan ;

Zhang, Yu ;

Sheng, Jiping ;

Shen, Lin ;

Zhao, Ruirui ;

Tang, Xuanming .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2016, 64 (43) :8200-8206

[7] Redox Regulation in Photosynthetic Organisms: Signaling, Acclimation, and Practical Implications [J].

Foyer, Christine H. ;

Noctor, Graham .

ANTIOXIDANTS & REDOX SIGNALING, 2009, 11 (04) :861-905

[8] Pawpaw Fruit Chilling Injury and Antioxidant Protection [J].

Galli, Federica ;

Archbold, Douglas D. ;

Pomper, Kirk W. .

JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, 2009, 134 (04) :466-471

[9]

Ge C., 2019, AIP CONF P, V2079

[10] Insights into the metabolism of membrane lipid fatty acids associated with chilling injury in post-harvest bell peppers [J].

Ge, Wanying ;

Kong, Ximan ;

Zhao, Yingbo ;

Wei, Baodong ;

Zhou, Qian ;

Ji, Shujuan .

FOOD CHEMISTRY, 2019, 295 :26-35

← 1 2 3 4 →