Remarkably selective biocompatible turn-on fluorescent probe for detection of Fe3+ in human blood samples and cells

被引:26
作者
Vishaka, V. H. [1 ]
Saxena, Manav [1 ]
Balakrishna, Geetha R. [1 ]
Latiyan, Sachin [2 ,3 ]
Jain, Shilpee [3 ]
机构
[1] Jain Univ, Ctr Nano & Mat Sci, Bangalore Rural 562112, India
[2] Natl Inst Technol, Met & Mat Engn, Tiruchirappalli 620015, India
[3] Indian Inst Sci, Ctr Biosyst Sci & Engn, Bangalore 560012, Karnataka, India
关键词
RHODAMINE-BASED CHEMOSENSOR; CHROMOGENIC DETECTION; ENERGY-TRANSFER; AQUEOUS-MEDIA; QUANTUM-DOT; ICP-MS; WATER; FE(III); RECOGNITION; AL(III);
D O I
10.1039/c9ra05256a
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The robust nature of a biocompatible fluorescent probe is demonstrated, by its detection of Fe3+ even after repeated rounds of quenching (reversibility) by acetate in real human blood samples and cells in vitro. Significantly trace levels of Fe3+ ions up to 8.2 nM could be detected, remaining unaffected by the existence of various other metal ions. The obtained results are validated by AAS and ICP-OES methods. A portable test strip is also fabricated for quick on field detection of Fe3+. As iron is a ubiquitous metal in cells and plays a prominent role in biological processes, the use of this probe to image Fe3+ in cells is a substantial development towards biosensing. Cytotoxicity studies also proved the nontoxic nature of this probe.
引用
收藏
页码:27439 / 27448
页数:10
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