Nanostructured zinc oxide film for urea sensor

被引：104

作者：

Ali, Azahar ^{[1
]}

Ansari, Anees A. ^{[1
]}

Kaushik, Ajeet ^{[1
]}

Solanki, Pratima R. ^{[1
]}

Barik, A. ^{[1
]}

Pandey, M. K. ^{[1
]}

Malhotra, B. D. ^{[1
]}

机构：

[1] Natl Phys Lab, Dept Sci & Technol, Ctr Biomol Elect, New Delhi 110012, India

来源：

MATERIALS LETTERS | 2009年 / 63卷 / 28期

关键词：

Nanostructured ZnO; Nanomaterials; Sensor; Electrochemical deposition; Enzyme; HORSERADISH-PEROXIDASE; ZNO; IMMOBILIZATION; MATRIX;

D O I：

10.1016/j.matlet.2009.08.038

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Nanostructured zinc oxide (Nano-ZnO) film has been electrochemically deposited onto indium-tin-oxide (ITO) coated glass plate to co-immobilized urease (Urs) and glutamate dehydrogenase (GLDH) for urea detection. The observed reflection planes corresponding to wurtzite ZnO nanoparticles (similar to 25 nm) in XRD diffraction pattern and UV-visible absorption band at 338 nm reveal the formation of Nano-ZnO. Urs-GLDH/Nano-ZnO/ITO bioelectrode shows high sensitivity for urea detection within 10-80 mg/dL and limit of detection as 13.5 mg/dL with regression coefficient as 0.994 and Michaelis-Menten constant (K-m, 6.1 mg/dL) indicating good affinity of Urs-GLDH to urea. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：2473 / 2475

页数：3

共 14 条

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