Fast responding biosensor for on-line determination of nitrate/nitrite in activated sludge

被引:53
作者
Larsen, LH
Damgaard, LR
Kjær, T
Stenstrom, T
Lynggaard-Jensen, A
Revsbech, NP
机构
[1] Aarhus Univ, Inst Biol Sci, Dept Microbial Ecol, DK-8000 Aarhus C, Denmark
[2] UPS, Danfoss Analyt, DK-6700 Sonderborg, Denmark
[3] Danish Water Qual Inst, DK-8000 Aarhus C, Denmark
关键词
wastewater; nitrate; biosensor; on-line measurements;
D O I
10.1016/S0043-1354(99)00423-6
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
A microscale biosensor for nitrate/nitrite was used for in-situ monitoring in an activated sludge plant. The biosensor is based on diffusion of nitrate/nitrite through a tip membrane into a dense mass of bacteria converting the ions into nitrous oxide with subsequent electrochemical detection. The biosensor signal was in very close agreement with the nitrate/nitrite concentration in the plant as analysed by conventional laboratory techniques. The concentration of the only interfering substance, nitrous oxide, was so low that it did not have any effect. The response time of the applied biosensors was less than 30 s, and the detection limit was less than 50 mu g N l(-1). Detachment of the ion-permeable membrane in the tip ol these first-generation biosensors limited the period with continuous monitoring to 5 days. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:2463 / 2468
页数:6
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