Synthesis and characterization of stearyl protamine and investigation of their complexes with DNA for gene delivery

被引：25
|
作者：
Liu, Jiong [1 ]
Guo, Shengrong [1 ]
Li, Zonghai [2 ]
Liu, Li [1 ]
Gu, Jianren [2 ]
机构：
[1] Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200240, Peoples R China
[2] Shanghai Jiao Tong Univ, Shanghai Canc Inst, Natl Lab Oncogenes & Related Genes, Shanghai 200240, Peoples R China
来源：
COLLOIDS AND SURFACES B-BIOINTERFACES | 2009年 / 73卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Non-viral; Gene delivery; Stearyl protamine; Complex; Transfection; VIRAL VECTORS; NANO DEVICE; IN-VITRO; THERAPY; POLYETHYLENIMINE; NANOPARTICLES; OCTAARGININE; TRANSFECTION; DENDRIMERS;
D O I：
10.1016/j.colsurfb.2009.04.026
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
A novel gene carrier, stearyl protamine (SP) was synthesized by the reaction of natural protamine with an N-hydroxysuccinimide (NHS) ester of stearic acid. This SP was characterized by FT-IR, stearyl content measurement and dynamic light scattering (DLS). Then, the SP was evaluated as a gene delivery carrier. The SP with higher stearyl group content(SP3) can self-assemble into aggregates with an average diameter of ca. 110nm in water and has relatively higher in vitro gene transfection efficiency than protamine and SP with lower stearyl group content. A possible mechanism is proposed for the formation of complex of SP/DNA. The SP also shows a low cytotoxicity similar to protamine. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码：36 / 41
页数：6
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