Synthesis and characterization of stearyl protamine and investigation of their complexes with DNA for gene delivery

被引：26

作者：

Liu, Jiong ^{[1
]}

Guo, Shengrong ^{[1
]}

Li, Zonghai ^{[2
]}

Liu, Li ^{[1
]}

Gu, Jianren ^{[2
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200240, Peoples R China

[2] Shanghai Jiao Tong Univ, Shanghai Canc Inst, Natl Lab Oncogenes & Related Genes, Shanghai 200240, Peoples R China

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2009年 / 73卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Non-viral; Gene delivery; Stearyl protamine; Complex; Transfection; VIRAL VECTORS; NANO DEVICE; IN-VITRO; THERAPY; POLYETHYLENIMINE; NANOPARTICLES; OCTAARGININE; TRANSFECTION; DENDRIMERS;

D O I：

10.1016/j.colsurfb.2009.04.026

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A novel gene carrier, stearyl protamine (SP) was synthesized by the reaction of natural protamine with an N-hydroxysuccinimide (NHS) ester of stearic acid. This SP was characterized by FT-IR, stearyl content measurement and dynamic light scattering (DLS). Then, the SP was evaluated as a gene delivery carrier. The SP with higher stearyl group content(SP3) can self-assemble into aggregates with an average diameter of ca. 110nm in water and has relatively higher in vitro gene transfection efficiency than protamine and SP with lower stearyl group content. A possible mechanism is proposed for the formation of complex of SP/DNA. The SP also shows a low cytotoxicity similar to protamine. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：36 / 41

页数：6

共 28 条

[1] Protamine sulfate protects exogenous DNA against nuclease degradation but is unable to improve the efficiency of bovine sperm mediated transgenesis [J].

Alderson, J ;

Wilson, B ;

Laible, G ;

Pfeffer, P ;

L'Huillier, P .

ANIMAL REPRODUCTION SCIENCE, 2006, 91 (1-2) :23-30

[2] Physical stability and in-vitro gene expression efficiency of nebulised lipid-peptide-DNA complexes [J].

Birchall, JC ;

Kellaway, IW ;

Gumbleton, M .

INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2000, 197 (1-2) :221-231

[3] Structure/function relationships of polyamidoamine/DNA dendrimers as gene delivery vehicles [J].

Braun, CS ;

Vetro, JA ;

Tomalia, DA ;

Koe, GS ;

Koe, JG ;

Middaugh, CR .

JOURNAL OF PHARMACEUTICAL SCIENCES, 2005, 94 (02) :423-436

[4] Protamine-induced condensation and decondensation of the same DNA molecule [J].

Brewer, LR ;

Corzett, M ;

Balhorn, R .

SCIENCE, 1999, 286 (5437) :120-123

[5] Gene delivery with synthetic (non viral) carriers [J].

Brown, MD ;

Schätzlein, AG ;

Uchegbu, IF .

INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2001, 229 (1-2) :1-21

[6] Dendrimers in gene delivery [J].

Dufès, C ;

Uchegbu, IF ;

Schätzlein, AG .

ADVANCED DRUG DELIVERY REVIEWS, 2005, 57 (15) :2177-2202

[7] Efficient synthesis and cell-transfection properties of a new multivalent cationic lipid for nonviral gene delivery [J].

Ewert, K ;

Ahmad, A ;

Evans, HM ;

Schmidt, HW ;

Safinya, CR .

JOURNAL OF MEDICINAL CHEMISTRY, 2002, 45 (23) :5023-5029

[8] Synthesis of a novel series of cationic lipids that can act as efficient gene delivery vehicles through systematic heterocyclic substitution of cholesterol derivatives [J].

Gao, H ;

Hui, KM .

GENE THERAPY, 2001, 8 (11) :855-863

[9] Purification and analysis of protamine [J].

Gill, Tom A. ;

Singer, Douglas S. ;

Thompson, John W. .

PROCESS BIOCHEMISTRY, 2006, 41 (08) :1875-1882

[10] Development of biomaterials for gene therapy [J].

Han, S ;

Mahato, RI ;

Sung, YK ;

Kim, SW .

MOLECULAR THERAPY, 2000, 2 (04) :302-317

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