Insertion sequences disrupting mgrB in carbapenem-resistant Klebsiella pneumoniae strains in Brazil

被引：20

作者：

da Silva, Daniely Martins ^{[1
]}

Faria-Junior, Celio ^{[2
]}

Nery, Danielly Rocha ^{[1
]}

de Oliveira, Pamela Maria ^{[1
]}

Rodrigues Silva, Lilian de Oliveira ^{[2
]}

Alves, Everton Giovanni ^{[2
]}

de Castro e Caldo Lima, Glaura Regina ^{[2
]}

Pereira, Alex Leite ^{[1
]}

机构：

[1] Univ Brasilia, Ctr Metropolitano, Campus Ceilandia,Conjunto a, BR-72220275 Brasilia, DF, Brazil

[2] Cent Lab Publ Hlth LACEN DF, SGAN 601, BR-70830010 Brasilia, DF, Brazil

来源：

JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE | 2021年 / 24卷

关键词：

Polymyxin resistance; mgrB; Insertion sequence;

D O I：

10.1016/j.jgar.2020.11.003

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Objectives: This study aimed to characterise insertional mutations disturbing themgrB gene in carbapenem-resistant Klebsiella pneumoniae (CRKp). Methods: A total of 118 clinical CRKp isolates were surveyed for polymyxin resistance and insertion sequence (IS) elements disruptingmgrB. Results: Of the 118 isolates, 78 (66.1%) displayed polymyxin resistance, of which 54% (42/78) hadmgrB::IS inserts. Sequencing analyses showed 13 insertion sites in mgrB. mgrB::ISSen4(IS3) was observed for the first time in CRKp. Conclusions: Ten different IS elements disruptedmgrB, with a predominance (76%) of IS5 sequences. (C) 2020 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy.

引用

页码：53 / 57

页数：5

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