Rhinovirus-induced modulation of gene expression in bronchial epithelial cells from subjects with asthma

被引：232

作者：

Bochkov, Y. A. ^{[1
]}

Hanson, K. M. ^{[1
]}

Keles, S. ^{[2
]}

Brockman-Schneider, R. A. ^{[1
]}

Jarjour, N. N. ^{[3
]}

Gern, J. E. ^{[1
,3
]}

机构：

[1] Univ Wisconsin, Dept Pediat, Madison, WI 53706 USA

[2] Univ Wisconsin, Dept Biostat & Med Informat, Madison, WI USA

[3] Univ Wisconsin, Dept Med, Madison, WI USA

来源：

MUCOSAL IMMUNOLOGY | 2010年 / 3卷 / 01期

基金：

美国国家卫生研究院;

关键词：

ALLERGIC AIRWAY INFLAMMATION; INFECTION; POLYMORPHISMS; ASSOCIATION; ACTIVATION; SEVERITY; ALPHA; SUSCEPTIBILITY; ATTENUATION; INHIBITION;

D O I：

10.1038/mi.2009.109

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Rhinovirus (RV) infections trigger asthma exacerbations. Genome-wide expression analysis of RV1A-infected primary bronchial epithelial cells from normal and asthmatic donors was performed to determine whether asthma is associated with a unique pattern of RV-induced gene expression. Virus replication rates were similar in cells from normal and asthmatic donors. Overall, RV downregulated 975 and upregulated 69 genes. Comparisons of transcriptional profiles generated from microarrays and confirmed by quantitative reverse transcription PCR and cluster analysis showed some up-and downregulated genes in asthma cells involved in immune responses (IL1B, IL1F9, IL24, and IFI44) and airway remodeling (LOXL2, MMP10, FN1). Notably, most of the asthma-related differences in RV-infected cells were also present in the cells before infection. These findings suggest that differences in RV-induced gene expression profiles of cells from normal and mild asthmatic subjects could affect the acute inflammatory response to RV, and subsequent airway repair and remodeling.

引用

页码：69 / 80

页数：12

共 48 条

[1] A Fra-1-dependent, matrix metalloproteinase driven EGFR activation promotes human lung epithelial cell motility and invasion [J].