Neutron diffraction analysis of Pseudomonas aeruginosa peptidyl-tRNA hydrolase 1

被引：3
作者：
McFeeters, Hana [1 ]
Vandavasi, Venu Gopal [2 ]
Weiss, Kevin L. [2 ]
Coates, Leighton [2 ]
McFeeters, Robert L. [1 ]
机构：
[1] Univ Alabama, Dept Chem, 301 Sparkman Dr, Huntsville, AL 35899 USA
[2] Oak Ridge Natl Lab, Biol & Soft Matter Div, 1 Bethel Valley Rd, Oak Ridge, TN 37831 USA
来源：
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2016年 / 72卷
关键词：
peptidyl-tRNA hydrolase 1; neutron diffraction; perdeuteration; Pseudomonas aeruginosa; antibiotic resistance; ESCHERICHIA-COLI; MINIGENE EXPRESSION; X-RAY; INHIBITION; CRYSTALLIZATION; PNEUMONIA; GROWTH;
D O I：
10.1107/S2053230X16001813
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Perdeuterated peptidyl-tRNA hydrolase 1 from Pseudomonas aeruginosa was crystallized for structural analysis using neutron diffraction. Crystals of perdeuterated protein were grown to 0.15 mm(3) in size using batch crystallization in 22.5% polyethylene glycol 4000, 100 mM Tris pH 7.5, 10% (v/v) isopropyl alcohol with a 20-molar excess of trilysine as an additive. Neutron diffraction data were collected from a crystal at room temperature using the MaNDi single-crystal diffractometer at Oak Ridge National Laboratory.
引用
收藏
页码：220 / 223
页数：4
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