Circular RNA circ_0000039 enhances gastric cancer progression through miR-1292-5p/DEK axis

被引:14
作者
Fan, Dengguo [1 ]
Wang, Changjiang [2 ]
Wang, Deyuan [3 ]
Zhang, Ning [4 ]
Yi, Tao [2 ]
机构
[1] Caoxian Peoples Hosp, Dept Gen Surg, Heze 274400, Shandong, Peoples R China
[2] Caoxian Peoples Hosp, Dept Gastroenterol, Fumin Ave,Qinghe Middle Rd, Heze 274400, Shandong, Peoples R China
[3] Caoxian Peoples Hosp, Dept Oncol, Heze 274400, Shandong, Peoples R China
[4] Caoxian Cty Hosp Shandong Prov, Heze 274400, Shandong, Peoples R China
关键词
Gastric cancer; circ_0000039; miR-1292-5p; DEK; proliferation; metastasis; DEK; INVASION; CARCINOMA; SURVIVAL;
D O I
10.3233/CBM-201754
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
BACKGROUND: Circular RNA (circRNA) is a class of non-coding RNA that is vital for regulating gene expression and biological functions. Mounting studies demonstrate that circRNA is crucial for human cancer development. However, the role of circ_0000039 in gastric cancer (GC) remains uncertain. METHODS: Normal human gastric tissues and GC tissue samples were collected, and quantitative real-time polymerase chain reaction (qRT-PCR) was employed to detect the expression levels of circ_0000039, miR-1292-5p, and DEK. GC cell lines with overexpression and low expression of circ_0000039 were constructed. Cell counting kit-8 (CCK-8), scratch healing and Transwell experiments were used to assess the function of circ_0000039 on the proliferation, migration and invasion of GC cells. Bioinformatics analysis and dual-luciferase reporter assays were employed to detect the targeting relationship between circ_0000039 and miR-1292-5p. RESULTS: Circ_0000039 expression was up-regulated in GC tissues and cell lines, and it was significantly related with poor differentiation of tumor tissues. In addition, circ_0000039 overexpression enhanced the proliferation, migration and invasion of GC cells, while circ_0000039 depletion inhibited these malignant biological behaviors. In terms of mechanism, it was found that circ_0000039 promoted the proliferation and progression of GC cells by adsorbing miR-1292-5p and up-regulating the expression of DEK. CONCLUSION: Circ_0000039 is a new oncogenic circRNA in GC, which regulates the miR-1292-5p/DEK axis to modulate the malignant biological behaviors of GC.
引用
收藏
页码:167 / 177
页数:11
相关论文
共 41 条
[1]   Global surveillance of cancer survival 1995-2009: analysis of individual data for 25 676 887 patients from 279 population-based registries in 67 countries (CONCORD-2) [J].
Allemani, Claudia ;
Weir, Hannah K. ;
Carreira, Helena ;
Harewood, Rhea ;
Spika, Devon ;
Wang, Xiao-Si ;
Bannon, Finian ;
Ahn, Jane V. ;
Johnson, Christopher J. ;
Bonaventure, Audrey ;
Marcos-Gragera, Rafael ;
Stiller, Charles ;
Azevedo e Silva, Gulnar ;
Chen, Wan-Qing ;
Ogunbiyi, Olufemi J. ;
Rachet, Bernard ;
Soeberg, Matthew J. ;
You, Hui ;
Matsuda, Tomohiro ;
Bielska-Lasota, Magdalena ;
Storm, Hans ;
Tucker, Thomas C. ;
Coleman, Michel P. .
LANCET, 2015, 385 (9972) :977-1010
[2]   Circ-BPTF promotes bladder cancer progression and recurrence through the miR-31-5p/RAB27A axis [J].
Bi, Junming ;
Liu, Hongwei ;
Cai, Zijian ;
Dong, Wei ;
Jiang, Ning ;
Yang, Meihua ;
Huang, Jian ;
Lin, Tianxin .
AGING-US, 2018, 10 (08) :1964-1976
[3]   Hsa_circ_101280 promotes hepatocellular carcinoma by regulating miR-375/JAK2 [J].
Cao, Shuang ;
Wang, Guohua ;
Wang, Jia ;
Li, Cheng ;
Zhang, Le .
IMMUNOLOGY AND CELL BIOLOGY, 2019, 97 (02) :218-228
[4]   Regulation of circRNA biogenesis [J].
Chen, Ling-Ling ;
Yang, Li .
RNA BIOLOGY, 2015, 12 (04) :381-388
[5]   Using circular RNA hsa_circ_0000190 as a new biomarker in the diagnosis of gastric cancer [J].
Chen, Shijun ;
Li, Tianwen ;
Zhao, Qianfu ;
Xiao, Bingxiu ;
Guo, Junming .
CLINICA CHIMICA ACTA, 2017, 466 :167-171
[6]   Circular RNAs: a new frontier in the study of human diseases [J].
Chen, Yonghua ;
Li, Cheng ;
Tan, Chunlu ;
Liu, Xubao .
JOURNAL OF MEDICAL GENETICS, 2016, 53 (06) :359-365
[7]   Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012 [J].
Ferlay, Jacques ;
Soerjomataram, Isabelle ;
Dikshit, Rajesh ;
Eser, Sultan ;
Mathers, Colin ;
Rebelo, Marise ;
Parkin, Donald Maxwell ;
Forman, David ;
Bray, Freddie .
INTERNATIONAL JOURNAL OF CANCER, 2015, 136 (05) :E359-E386
[8]   Identification of circRNA-miRNA-mRNA regulatory network in gastric cancer by analysis of microarray data [J].
Guan, Yong-jun ;
Ma, Jian-ying ;
Song, Wei .
CANCER CELL INTERNATIONAL, 2019, 19 (1)
[9]   Noncoding Effects of Circular RNA CCDC66 Promote Colon Cancer Growth and Metastasis [J].
Hsiao, Kuei-Yang ;
Lin, Ya-Chi ;
Gupta, Sachin Kumar ;
Chang, Ning ;
Yen, Laising ;
Sun, H. Sunny ;
Tsai, Shaw-Jenq .
CANCER RESEARCH, 2017, 77 (09) :2339-2350
[10]   Circular RNA hsa_circ_0000745 may serve as a diagnostic marker for gastric cancer [J].
Huang, Mei ;
He, Yi-Ren ;
Liang, Li-Chuan ;
Huang, Qiang ;
Zhu, Zhi-Qiang .
WORLD JOURNAL OF GASTROENTEROLOGY, 2017, 23 (34) :6330-6338