Transcriptome analysis reveals underlying immune response mechanism of fungal (Penicillium oxalicum) disease in Gastrodia elata Bl. f. glauca S. chow (Orchidaceae)

被引：13

作者：

Wang, Yanhua ^{[1
]}

Gao, Yugang ^{[1
]}

Zang, Pu ^{[1
]}

Xu, Yue ^{[1
]}

机构：

[1] Jilin Agr Univ, Coll Chinese Med Mat, Changchun 130118, Peoples R China

来源：

BMC PLANT BIOLOGY | 2020年 / 20卷 / 01期

关键词：

Gastrodia elata Bl. f. glauca S. chow; Orchidaceae; Transcriptome; Fungal disease; immune response; Transcription factors; Changbai Mountain area; POSITIVE REGULATOR; STRESS TOLERANCE; JASMONIC ACID; RESISTANCE; EXPRESSION; RICE; GENES; SUSCEPTIBILITY; DEFICITS; PROTEIN;

D O I：

10.1186/s12870-020-02653-4

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Background: Gastrodia elata Bl. f. glauca S. Chow is a medicinal plant G. elata f. glauca is unavoidably infected by pathogens in their growth process. In previous work, we have successfully isolated and identified Penicillium oxalicum from fungal diseased tubers of G. elata f. glauca. As a widespread epidemic, this fungal disease seriously affected the yield and quality of G. elata f. glauca. We speculate that the healthy G. elata F. glauca might carry resistance genes, which can resist against fungal disease. In this study, healthy and fungal diseased mature tubers of G. elata f. glauca from Changbai Mountain area were used as experimental materials to help us find potential resistance genes against the fungal disease. Results: A total of 7540 differentially expressed Unigenes (DEGs) were identified (FDR < 0.01, log2FC > 2). The current study screened 10 potential resistance genes. They were attached to transcription factors (TFs) in plant hormone signal transduction pathway and plant pathogen interaction pathway, including WRKY22, GH3, TlFY/JAZ, ERF1, WRKY33, TGA In addition, four of these genes were closely related to jasmonic acid signaling pathway. Conclusions: The immune response mechanism of fungal disease in G. elata f. glauca is a complex biological process, involving plant hormones such as ethylene, jasmonic acid, salicylic acid and disease-resistant transcription factors such as WRKY, TGA.

引用

页数：17

共 92 条

[1] The ERF transcription factor MdERF38 promotes drought stress-induced anthocyanin biosynthesis in apple [J].

An, Jian-Ping ;

Zhang, Xiao-Wei ;

Bi, Si-Qi ;

You, Chun-Xiang ;

Wang, Xiao-Fei ;

Hao, Yu-Jin .

PLANT JOURNAL, 2020, 101 (03) :573-589

[2]

[Anonymous], 2010, GENOME BIOL, DOI DOI 10.1186/gb-2010-11-10-r106

[3] Gene Ontology: tool for the unification of biology [J].

Ashburner, M ;

Ball, CA ;

Blake, JA ;

Botstein, D ;

Butler, H ;

Cherry, JM ;

Davis, AP ;

Dolinski, K ;

Dwight, SS ;

Eppig, JT ;

Harris, MA ;

Hill, DP ;

Issel-Tarver, L ;

Kasarskis, A ;

Lewis, S ;

Matese, JC ;

Richardson, JE ;

Ringwald, M ;

Rubin, GM ;

Sherlock, G .

NATURE GENETICS, 2000, 25 (01) :25-29

[4] Genome sequencing of the Australian wild diploid species Gossypium australe highlights disease resistance and delayed gland morphogenesis [J].

Cai, Yingfan ;

Cai, Xiaoyan ;

Wang, Qinglian ;

Wang, Ping ;

Zhang, Yu ;

Cai, Chaowei ;

Xu, Yanchao ;

Wang, Kunbo ;

Zhou, Zhongli ;

Wang, Chenxiao ;

Geng, Shuaipeng ;

Li, Bo ;

Dong, Qi ;

Hou, Yuqing ;

Wang, Heng ;

Ai, Peng ;

Liu, Zhen ;

Yi, Feifei ;

Sun, Minshan ;

An, Guoyong ;

Cheng, Jieru ;

Zhang, Yuanyuan ;

Shi, Qian ;

Xie, Yuanhui ;

Shi, Xinying ;

Chang, Ying ;

Huang, Feifei ;

Chen, Yun ;

Hong, Shimiao ;

Mi, Lingyu ;

Sun, Quan ;

Zhang, Lin ;

Zhou, Baoliang ;

Peng, Renhai ;

Zhang, Xiao ;

Liu, Fang .

PLANT BIOTECHNOLOGY JOURNAL, 2020, 18 (03) :814-828

[5] Inhibition of multiple defense responsive pathways by CaWRKY70 transcription factor promotes susceptibility in chickpea under Fusarium oxysporum stress condition [J].

Chakraborty, Joydeep ;

Sen, Senjuti ;

Ghosh, Prithwi ;

Jain, Akansha ;

Das, Sampa .

BMC PLANT BIOLOGY, 2020, 20 (01)

[6] Gastrodia elata Bl. Attenuated Learning Deficits Induced by Forced- Swimming Stress in the Inhibitory Avoidance Task and Morris Water Maze [J].

Chen, Pei-Ju ;

Liang, Keng-Chen ;

Lin, Hui-Chen ;

Hsieh, Ching-Liang ;

Su, Kuan-Pin ;

Hung, Mei-Chu ;

Sheen, Lee-Yan .

JOURNAL OF MEDICINAL FOOD, 2011, 14 (06) :610-617

[7] Molecular cloning and functional analysis of 4-Coumarate:CoA ligase 4(4CL-like 1)from Fraxinus mandshurica and its role in abiotic stress tolerance and cell wall synthesis [J].

Chen, Xiaohui ;

Wang, Hengtao ;

Li, Xiaoyi ;

Ma, Kai ;

Zhan, Yaguang ;

Zeng, Fansuo .

BMC PLANT BIOLOGY, 2019, 19 (1)

[8] The transcriptional reprograming and functional identification of WRKY family members in pepper's response to Phytophthora capsici infection [J].

Cheng, Wei ;

Jiang, Yan ;

Peng, Jiangtao ;

Guo, Jianwen ;

Lin, Menglan ;

Jin, Chengting ;

Huang, Jinfeng ;

Tang, Weiqi ;

Guan, Deyi ;

He, Shuilin .

BMC PLANT BIOLOGY, 2020, 20 (01)

[9]

Chinese Pharmacopoeia Commission, Pharmacopoeia of the People's Republic of China

[10] Identification of TIFY/JAZ family genes in Solanum lycopersicum and their regulation in response to abiotic stresses [J].

Chini, Andrea ;

Ben-Romdhane, Walid ;

Hassairi, Afif ;

Aboul-Soud, Mourad A. M. .

PLOS ONE, 2017, 12 (06)

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