Establishing a mass spectrometry-based system for rapid detection of SARS-CoV-2 in large clinical sample cohorts

被引：94

作者：

Morais Cardozo, Karina Helena ^{[1
]}

Lebkuchen, Adriana ^{[1
]}

Okai, Guilherme Goncalves ^{[1
]}

Schuch, Rodrigo Andrade ^{[1
]}

Viana, Luciana Godoy ^{[1
]}

Olive, Aline Nogueira ^{[1
]}

Lazari, Carolina dos Santos ^{[2
]}

Fraga, Ana Maria ^{[1
]}

Hernandes Granato, Celso Francisco ^{[2
]}

Tostes Pintao, Maria Carolina ^{[1
]}

Carvalho, Valdemir Melechco ^{[1
]}

机构：

[1] Fleury Grp, Div Res & Dev, BR-04344070 Sao Paulo, SP, Brazil

[2] Fleury Grp, Div Infect Dis, BR-04344070 Sao Paulo, SP, Brazil

来源：

NATURE COMMUNICATIONS | 2020年 / 11卷 / 01期

关键词：

LIQUID-CHROMATOGRAPHY; TARGETED PROTEOMICS; PEPTIDES; PROTEIN; QUANTIFICATION; CARRYOVER; STORAGE;

D O I：

10.1038/s41467-020-19925-0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is pressing public health systems around the world, and large population testing is a key step to control this pandemic disease. Here, we develop a high-throughput targeted proteomics assay to detect SARS-CoV-2 nucleoprotein peptides directly from nasopharyngeal and oropharyngeal swabs. A modified magnetic particle-based proteomics approach implemented on a robotic liquid handler enables fully automated preparation of 96 samples within 4hours. A TFC-MS system allows multiplexed analysis of 4 samples within 10min, enabling the processing of more than 500 samples per day. We validate this method qualitatively (Tier 3) and quantitatively (Tier 1) using 985 specimens previously analyzed by real-time RT-PCR, and detect up to 84% of the positive cases with up to 97% specificity. The presented strategy has high sample stability and should be considered as an option for SARS-CoV-2 testing in large populations.

引用

页数：13

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