Observation of liquid-liquid phase separation for eye lens γS-crystallin

被引:60
作者
Annunziata, O
Ogun, O
Benedek, GB [1 ]
机构
[1] MIT, Dept Phys, Ctr Mat Sci & Engn, Cambridge, MA 02139 USA
[2] MIT, Mat Proc Ctr, Cambridge, MA 02139 USA
关键词
protein; cataract; polyethylene glycol; phase transition; partitioning;
D O I
10.1073/pnas.242746499
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
gammaS-crystallin (gammaS) is an important human and bovine eye lens protein involved in maintaining the transparency of the eye. By adding small amounts of polyethylene glycol (PEG) to the binary aqueous bovine gammaS solutions, we have observed liquid-liquid phase separation (LLPS) at -8degreesC and revealed that, in the binary gammaS-water system, this phase transition would occur at -28degreesC. We have measured both the effect of PEG concentration on the LLPS temperature and protein/PEG partitioning between the two liquid coexisting phases. We use our measurements of protein/PEG partitioning to determine the nature and the magnitude of the gammaS-PEG interactions and to quantitatively assess the effectiveness of PEG as a crystallizing agent for gammaS. We use our measurements of LLPS temperature as a function of protein and PEG concentration to successfully determine the location of the critical point for the binary gammaS-water system. This phase transition cannot be observed in the absence of PEG because it is inaccessible due to the freezing of the system. Our findings indicate that the effective interactions between gammaS molecules in the binary gammaS-water system are attractive. We compare the magnitude of the attraction found for gammaS with the results obtained for the other gamma-crystallins for which the critical temperature is located above the freezing point of the system. This work suggests that PEG can be used to reveal the existence of LLPS for a much wider range of binary protein-water systems than known previously.
引用
收藏
页码:970 / 974
页数:5
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