Investigation on hyperin-cetyltrimethylammonium bromide-fibronectin system by resonance light-scattering technique

被引：13
作者：
Gao, Dejiang
Tian, Yuan
Liang, Fanghui
Bi, Shuyun
Li, Tiechun
Chen, Yanhua
Zhang, Hanqi
Yu, Aimin [1 ]
机构：
[1] Jilin Univ, Coll Chem, Changchun 130012, Peoples R China
[2] Jinan Univ, Coll Life Sci & Technol, Guangzhou 510632, Guangdong, Peoples R China
来源：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2007年 / 66卷 / 01期
关键词：
fibronectin; CTMAB; hyperin; resonance light-scattering technique;
D O I：
10.1016/j.saa.2006.02.020
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
A simple, highly sensitive assay for fibronectin (Fn) was reported using resonance light-scattering (RLS) technique based on the enhanced RLS intensity of hyperin-cetyltrimethylammonium bromide (CTMAB)-Fn system. The interaction system of hyperin-CTMAB-Fn was investigated using spectral methods. Mechanistic investigations show that the main reason of the enhanced RLS intensity of Fn is the formation of three-component complex (hyperin-CTMAB-Fn), in which CTMAB acts as a bridge between hyperin and Fn. The effects of pH, surfactant, concentration of CTMAB and hyperin, incubation time and foreign substances on the enhancement of RLS intensity were studied. Under the optimum conditions, the enhancement of RLS intensity is in proportion to the concentration of Fn in the range of 1.9-248 ng/ml. The synthetic samples containing Fn were analyzed and results obtained were satisfied. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：52 / 57
页数：6
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