Molecular Detection of Candidatus Bartonella mayotimonensis in North American Bats

被引：30

作者：

Lilley, Thomas M. ^{[1
,2
]}

Wilson, Cali A. ^{[1
]}

Bernard, Riley F.

Willcox, Emma V. ^{[3
]}

Vesterinen, Eero J. ^{[3
,4
]}

Webber, Quinn M. R. ^{[5
]}

Kurpiers, Laura ^{[1
]}

Prokkola, Jenni M. ^{[1
]}

Ejotre, Imran ^{[1
]}

Kurta, Allen ^{[6
]}

Field, Kenneth A. ^{[1
]}

Reeder, DeeAnn M. ^{[1
]}

Pulliainen, Arto T. ^{[7
]}

机构：

[1] Bucknell Univ, Dept Biol, Lewisburg, PA 17837 USA

[2] Univ Turku, Zool Museum, Biodivers Unit, Turku, Finland

[3] Univ Tennessee, Dept Forestry Wildlife & Fisheries, Knoxville, TN USA

[4] Univ Helsinki, Dept Agr Sci, Helsinki, Finland

[5] Mem Univ Newfoundland, Cognit & Behav Ecol CABE, St John, NF, Canada

[6] Eastern Michigan Univ, Dept Biol, Ypsilanti, MI 48197 USA

[7] Univ Turku, Inst Biomed, Kiinamyllynkatu 10, FIN-20520 Turku, Finland

来源：

VECTOR-BORNE AND ZOONOTIC DISEASES | 2017年 / 17卷 / 04期

关键词：

Bartonella; bats; Chiroptera; endocarditis; KELLEYI ACARI; SPP; IDENTIFICATION; ARGASIDAE; RESERVOIR; GENE;

D O I：

10.1089/vbz.2016.2080

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Candidatus Bartonella mayotimonensis was detected in 2010 from an aortic valve sample of a patient with endocarditis from Iowa, the United States of America. The environmental source of the potentially new endocarditis-causing Bartonella remained elusive. We set out to study the prevalence and diversity of bat-associated Bartonella in North America. During 2015, mist nets and harp traps were used to capture 92 bats belonging to two species: little brown myotis (Myotis lucifugus Le Conte 1831, n = 73) and the gray myotis (M. grisescens A.H. Howell 1909, n = 19) in Kentucky, Michigan, Pennsylvania, and Tennessee. DNA preparations of peripheral blood samples from bats were subjected to a three-marker ( gltA, rpoB, and intergenic spacer region [ISR]) multilocus sequence analysis. Sequence-verified gltA-positive PCR amplicons were obtained from nine samples. Three sequences were 99.7-100% identical with the gltA sequence of the Iowa endocarditis patient strain. Analysis of rpoB and ISR sequences demonstrated that one little brown myotis sample from the Upper Peninsula of Michigan contained Bartonella DNA, with 100% sequence identity with the Iowa endocarditis patient strain DNA. It appears possible that bats are a reservoir of Candidatus Bartonella mayotimonensis in North America.

引用

页码：243 / 246

页数：4

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