The morphologies of breast cancer cell lines in three-dimensional assays correlate with their profiles of gene expression

被引：815

作者：

Kenny, Paraic A. ^{[1
]}

Lee, Genee Y. ^{[1
]}

Myers, Connie A. ^{[1
]}

Neve, Richard M. ^{[1
]}

Semeiks, Jeremy R. ^{[1
]}

Spellman, Paul T. ^{[1
]}

Lorenz, Katrin ^{[1
]}

Lee, Eua H. ^{[1
]}

Barcellos-Hoff, Mary Helen ^{[1
]}

Petersen, Ole W. ^{[2
]}

Gray, Joe W. ^{[1
]}

Bissell, Mina J. ^{[1
]}

机构：

[1] Univ Calif Berkeley, Div Life Sci, Lawrence Berkeley Lab, Berkeley, CA 94720 USA

[2] Univ Copenhagen, Panum Inst, Struct Cell Biol Unit, Inst Med Anat, DK-2200 Copenhagen N, Denmark

来源：

MOLECULAR ONCOLOGY | 2007年 / 1卷 / 01期

关键词：

Breast cancer; 3D culture; Extracellular matrix; Gene expression profiling; Signal transduction;

D O I：

10.1016/j.molonc.2007.02.004

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

3D cell cultures are rapidly becoming the method of choice for the physiologically relevant modeling of many aspects of non-malignant and malignant cell behavior ex vivo. Nevertheless, only a limited number of distinct cell types have been evaluated in this assay to date. Here we report the first large scale comparison of the transcriptional profiles and 3D cell culture phenotypes of a substantial panel of human breast cancer cell lines. Each cell line adopts a colony morphology of one of four main classes in 3D culture. These morphologies reflect, at least in part, the underlying gene expression profile and protein expression patterns of the cell lines, and distinct morphologies were also associated with tumor cell invasiveness and with cell lines originating from metastases. We further demonstrate that consistent differences in genes encoding signal transduction proteins emerge when even tumor cells are cultured in 3D microenvironments. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：84 / 96

页数：13

共 64 条

[41] A collection of breast cancer cell lines for the study of functionally distinct cancer subtypes [J].

Neve, Richard M. ;

Chin, Koei ;

Fridlyand, Jane ;

Yeh, Jennifer ;

Baehner, Frederick L. ;

Fevr, Tea ;

Clark, Laura ;

Bayani, Nora ;

Coppe, Jean-Philippe ;

Tong, Frances ;

Speed, Terry ;

Spellman, Paul T. ;

DeVries, Sandy ;

Lapuk, Anna ;

Wang, Nick J. ;

Kuo, Wen-Lin ;

Stilwell, Jackie L. ;

Pinkel, Daniel ;

Albertson, Donna G. ;

Waldman, Frederic M. ;

McCormick, Frank ;

Dickson, Robert B. ;

Johnson, Michael D. ;

Lippman, Marc ;

Ethier, Stephen ;

Gazdar, Adi ;

Gray, Joe W. .

CANCER CELL, 2006, 10 (06) :515-527

[42] Serine phosphorylation-regulated ubiquitination and degradation of beta-catenin [J].

Orford, K ;

Crockett, C ;

Jensen, JP ;

Weissman, AM ;

Byers, SW .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (40) :24735-24738

[43] Transforming properties of YAP, a candidate oncogene on the chromosome 11q22 amplicon [J].

Overholtzer, Michael ;

Zhang, Jianmin ;

Smolen, Gromoslaw A. ;

Muir, Beth ;

Li, Wenmei ;

Sgroi, Dennis C. ;

Deng, Chu-Xia ;

Brugge, Joan S. ;

Haber, Daniel A. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (33) :12405-12410

[44] β1 integrin inhibitory antibody induces apoptosis of breast cancer cells, inhibits growth, and distinguishes malignant from normal phenotype in three dimensional cultures and in vivo [J].

Park, CC ;

Zhang, H ;

Paravicini, M ;

Gray, JW ;

Baehner, F ;

Park, CJ ;

Bissell, MJ .

CANCER RESEARCH, 2006, 66 (03) :1526-1535

[45] Distinctive gene expression patterns in human mammary epithelial cells and breast cancers [J].

Perou, CM ;

Jeffrey, SS ;

Van de Rijn, M ;

Rees, CA ;

Eisen, MB ;

Ross, DT ;

Pergamenschikov, A ;

Williams, CF ;

Zhu, SX ;

Lee, JCF ;

Lashkari, D ;

Shalon, D ;

Brown, PO ;

Botstein, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (16) :9212-9217

[46] Molecular portraits of human breast tumours [J].

Perou, CM ;

Sorlie, T ;

Eisen, MB ;

van de Rijn, M ;

Jeffrey, SS ;

Rees, CA ;

Pollack, JR ;

Ross, DT ;

Johnsen, H ;

Akslen, LA ;

Fluge, O ;

Pergamenschikov, A ;

Williams, C ;

Zhu, SX ;

Lonning, PE ;

Borresen-Dale, AL ;

Brown, PO ;

Botstein, D .

NATURE, 2000, 406 (6797) :747-752

[47] INTERACTION WITH BASEMENT-MEMBRANE SERVES TO RAPIDLY DISTINGUISH GROWTH AND DIFFERENTIATION PATTERN OF NORMAL AND MALIGNANT HUMAN BREAST EPITHELIAL-CELLS [J].

PETERSEN, OW ;

RONNOVJESSEN, L ;

HOWLETT, AR ;

BISSELL, MJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (19) :9064-9068

[48] RETRACTED: Genomic signatures to guide the use of chemotherapeutics (Retracted article. See vol. 17, pg. 135, 2011) [J].

Potti, Anil ;

Dressman, Holly K. ;

Bild, Andrea ;

Riedel, Richard F. ;

Chan, Gina ;

Sayer, Robyn ;

Cragun, Janiel ;

Cottrill, Hope ;

Kelley, Michael J. ;

Petersen, Rebecca ;

Harpole, David ;

Marks, Jeffrey ;

Berchuck, Andrew ;

Ginsburg, Geoffrey S. ;

Febbo, Phillip ;

Lancaster, Johnathan ;

Nevins, Joseph R. .

NATURE MEDICINE, 2006, 12 (11) :1294-1300

[49] Integrins and EGFR coordinately regulate the pro-apoptotic protein Bim to prevent anoikis [J].

Reginato, MJ ;

Mills, KR ;

Paulus, JK ;

Lynch, DK ;

Sgroi, DC ;

Debnath, J ;

Muthuswamy, SK ;

Brugge, JS .

NATURE CELL BIOLOGY, 2003, 5 (08) :733-740

[50] EXTRACELLULAR MATRIX-DEPENDENT TISSUE-SPECIFIC GENE-EXPRESSION IN MAMMARY EPITHELIAL-CELLS REQUIRES BOTH PHYSICAL AND BIOCHEMICAL SIGNAL-TRANSDUCTION [J].

ROSKELLEY, CD ;

DESPREZ, PY ;

BISSELL, MJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (26) :12378-12382

← 1 2 3 4 5 6 7 →