Isolation and culture of porcine primary fetal progenitors and neurons from the developing dorsal telencephalon

被引:3
作者
Aubid, Niroch Nawzad [1 ]
Liu, Yong [1 ]
Vidal, Juan Miguel Peralvo [1 ]
Hall, Vanessa Jane [1 ]
机构
[1] Univ Copenhagen, Fac Hlth Sci, Dept Vet & Anim Sci, Groennegaardsvej 7, DK-1870 Frederiksberg C, Denmark
关键词
Brain; cell culture techniques; neurons; BRAIN-CELLS; MECHANISMS; DIFFERENTIATION; GROWTH;
D O I
10.4142/jvs.2019.20.e3
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
The development of long-term surviving fetal cell cultures from primary cell tissue from the developing brain is important for facilitating studies investigating neural development and for modelling neural disorders and brain congenital defects. The field faces current challenges in co-culturing both progenitors and neurons long-term. Here, we culture for the first time, porcine fetal cells from the dorsal telencephalon at embryonic day (E) 50 and E60 in conditions that promoted both the survival of progenitor cells and young neurons. We applied a novel protocol designed to collect, isolate and promote survival of both progenitors and young neurons. Herein, we used a combination of low amount of fetal bovine serum, together with pro-survival factors, including basic fibroblast growth factor and retinoic acid, together with arabinofuranosylcytosine and could maintain progenitors and facilitate in vitro differentiation into calbindin 1+ neurons and reelin+ interneurons for a period of 7 days. Further improvements to the protocol that might extend the survival of the fetal primary neural cells would be beneficial. The development of new porcine fetal culture methods is of value for the field, given the pig's neuroanatomical and developmental similarities to the human brain.
引用
收藏
页数:13
相关论文
共 32 条
[1]   Sonographic prenatal diagnosis of central nervous system abnormalities [J].
Aubry, MC ;
Aubry, JP ;
Dommergues, M .
CHILDS NERVOUS SYSTEM, 2003, 19 (7-8) :391-402
[2]   An improved method for dissociation and aggregate culture of human fetal brain cells in serum-free medium [J].
Barnea, A ;
Roberts, J .
BRAIN RESEARCH PROTOCOLS, 1999, 4 (02) :156-164
[3]   The telencephalon of the Gottingen minipig, cytoarchitecture and cortical surface anatomy [J].
Bjarkam, Carsten R. ;
Glud, Andreas N. ;
Orlowski, Dariusz ;
Sorensen, Jens Christian H. ;
Palomero-Gallagher, Nicola .
BRAIN STRUCTURE & FUNCTION, 2017, 222 (05) :2093-2114
[4]   Cross-Species Analysis of Gene Expression and Function in Prefrontal Cortex, Hippocampus and Striatum [J].
Chen, Wei ;
Xia, Xiayu ;
Song, Nan ;
Wang, Ying ;
Zhu, Hua ;
Deng, Wei ;
Kong, Qi ;
Pan, Xianmin ;
Qin, Chuan .
PLOS ONE, 2016, 11 (10)
[5]   Brain Growth of the Domestic Pig (Sus scrofa) from 2 to 24 Weeks of Age: A Longitudinal MRI Study [J].
Conrad, Matthew S. ;
Dilger, Ryan N. ;
Johnson, Rodney W. .
DEVELOPMENTAL NEUROSCIENCE, 2012, 34 (04) :291-298
[6]   Microstructure of the neocortex: Comparative aspects [J].
DeFelipe, J ;
Alonso-Nanclares, L ;
Arellano, JI .
JOURNAL OF NEUROCYTOLOGY, 2002, 31 (3-5) :299-316
[7]   Neural Stem Cells to Cerebral Cortex: Emerging Mechanisms Regulating Progenitor Behavior and Productivity [J].
Dwyer, Noelle D. ;
Chen, Bin ;
Chou, Shen-Ju ;
Hippenmeyer, Simon ;
Nguyen, Laurent ;
Ghashghaei, H. Troy .
JOURNAL OF NEUROSCIENCE, 2016, 36 (45) :11394-11401
[8]   First and second trimester screening for fetal structural anomalies [J].
Edwards, Lindsay ;
Hui, Lisa .
SEMINARS IN FETAL & NEONATAL MEDICINE, 2018, 23 (02) :102-111
[9]  
French Leon, 2011, Front Neuroinform, V5, P12, DOI 10.3389/fninf.2011.00012
[10]   ACTIVITY AND METABOLISM-RELATED Ca2+ AND MITOCHONDRIAL DYNAMICS IN CO-CULTURED HUMAN FETAL CORTICAL NEURONS AND ASTROCYTES [J].
Fu, W. ;
Ruangkittisakul, A. ;
Mactavish, D. ;
Baker, G. B. ;
Ballanyi, K. ;
Jhamandas, J. H. .
NEUROSCIENCE, 2013, 250 :520-535