Two complement fixing pectic polysaccharides from pedicel of Lycium barbarum L. promote cellular antioxidant defense

被引：22
作者：
Yao, Ruyu [1 ,2 ]
Huang, Chao [1 ]
Chen, Xingfu [3 ]
Yin, Zhongqiong [1 ]
Fu, Yuping [1 ]
Li, Lixia [1 ]
Feng, Bin [4 ]
Song, Xu [1 ]
He, Changliang [1 ]
Yue, Guizhou [5 ]
Jing, Bo [1 ]
Lv, Cheng [1 ]
Su, Gang [1 ]
Ye, Gang [1 ]
Zou, Yuanfeng [1 ]
机构：
[1] Sichuan Agr Univ, Nat Med Res Ctr, Coll Vet Med, Wenjiang 611130, Peoples R China
[2] Univ Zurich, Dept Systemat & Evolutionary Bot, Zollikerstr 107, CH-8008 Zurich, Switzerland
[3] Sichuan Agr Univ, Coll Agron, Minist Agr, Key Lab Crop Ecophysiol & Farming Syst Southwest, Wenjiang 611130, Peoples R China
[4] Sichuan Agr Univ, Anim Nutr Inst, Wenjiang 611130, Peoples R China
[5] Sichuan Agr Univ, Coll Sci, Dept Appl Chem, Wenjiang 611130, Peoples R China
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2018年 / 112卷
基金：
中国博士后科学基金;
关键词：
Lycium barbarum pedicel; Polysaccharides; Immunomodulating activity; STRUCTURAL-CHARACTERIZATION; HYPOGLYCEMIC ACTIVITY; OPILIA-CELTIDIFOLIA; OXIDATIVE STRESS; GOJI FRUITS; EXTRACTION; LEAVES; PURIFICATION; HEALTH; POLYPHENOLS;
D O I：
10.1016/j.ijbiomac.2018.01.207
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Purification, characterization and biological activities of polysaccharides from Lycium barbarism pedicel were investigated in this study. Two polysaccharides, PLEIP-I-I and PLBP-II-I, were obtained from water extracts by anion exchange chromatography and gel filtration. Structural elucidation based on IR. H-1 NMR, and C-13 NMR spectra indicated that these two fractions were typical pectic polysaccharides, with homogalacturonan and rhamnogalacturonan type I regions and arabinogalactan side chains, and some of the galacturonic acid units were methyl esterified. Both fractions exhibited potent complement fixating activity and pro-antioxidant defense capacity, and those two fractions showed different activities. The higher complement fixation activity was obtained in fraction PLBP-I-I, while the higher pro-antioxidant defense capacity was obtained in fraction PLBP-II-I, which may be due to the structural differences between those two fractions. Thus, the pedicel of L. barbarum could be used as a potential source for natural immunomodulator and antioxidant. (C) 2018 Elsevier B.V. All rights reserved.
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收藏
页码：356 / 363
页数：8
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