Production and incorporation of N-15, C-13, H-2 (H-1-delta 1 methyl) isoleucine into proteins for multidimensional NMR studies

被引：219
作者：
Gardner, KH
Kay, LE
机构：
[1] UNIV TORONTO,DEPT MED GENET,TORONTO,ON M5S 1A8,CANADA
[2] UNIV TORONTO,DEPT MICROBIOL BIOCHEM & CHEM,TORONTO,ON M5S 1A8,CANADA
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 1997年 / 119卷 / 32期
关键词：
D O I：
10.1021/ja9706514
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
[No abstract available]
引用
收藏
页码：7599 / 7600
页数：2
相关论文
共 30 条
[11]   C-13 LINE NARROWING BY H-2 DECOUPLING IN H-2/C-13/N-15-ENRICHED PROTEINS - APPLICATION TO TRIPLE-RESONANCE 4D J-CONNECTIVITY OF SEQUENTIAL AMIDES [J].
GRZESIEK, S ;
ANGLISTER, J ;
REN, H ;
BAX, A .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (10) :4369-4370
[12]   4-DIMENSIONAL N-15-SEPARATED NOESY OF SLOWLY TUMBLING PERDEUTERATED N-15-ENRICHED PROTEINS - APPLICATION TO HIV-1 NEF [J].
GRZESIEK, S ;
WINGFIELD, P ;
STAHL, S ;
KAUFMAN, JD ;
BAX, A .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1995, 117 (37) :9594-9595
[13]  
HERINGA J, 1992, COMPUT APPL BIOSCI, V8, P599
[14]   SURFACE, SUBUNIT INTERFACES AND INTERIOR OF OLIGOMERIC PROTEINS [J].
JANIN, J ;
MILLER, S ;
CHOTHIA, C .
JOURNAL OF MOLECULAR BIOLOGY, 1988, 204 (01) :155-164
[15]   HIGH-RESOLUTION NUCLEAR MAGNETIC RESONANCE SPECTRA OF SELECTIVELY DEUTERATED STAPHYLOCOCCAL NUCLEASE [J].
MARKLEY, JL ;
PUTTER, I ;
JARDETZKY, O .
SCIENCE, 1968, 161 (3847) :1249-+
[16]   Incorporation of H-1/C-13/N-15-{Ile, Leu, Val} into a perdeuterated, N-15-labeled protein: Potential in structure determination of large proteins by NMR [J].
Metzler, WJ ;
Wittekind, M ;
Goldfarb, V ;
Mueller, L ;
Farmer, BT .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (28) :6800-6801
[17]  
MUHANDIRAM DR, 1993, J BIOMOL NMR, V3, P463
[18]   An approach to the structure determination of larger proteins using triple resonance NMR experiments in conjunction with random fractional deuteration [J].
Nietlispach, D ;
Clowes, RT ;
Broadhurst, RW ;
Ito, Y ;
Keeler, J ;
Kelly, M ;
Ashurst, J ;
Oschkinat, H ;
Domaille, PJ ;
Laue, ED .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (02) :407-415
[19]   H-1-NMR STUDIES OF DEUTERATED RIBONUCLEASE HI SELECTIVELY LABELED WITH PROTONATED AMINO-ACIDS [J].
ODA, Y ;
NAKAMURA, H ;
YAMAZAKI, T ;
NAGAYAMA, K ;
YOSHIDA, M ;
KANAYA, S ;
IKEHARA, M .
JOURNAL OF BIOMOLECULAR NMR, 1992, 2 (02) :137-147
[20]  
PASCAL SM, UNPUB
123