Melatonin and/or rowatinex attenuate streptozotocin-induced diabetic renal injury in rats

被引：15
作者：
Motawi, Tarek K. [1 ]
Ahmed, Samia A. [2 ]
Hamed, Manal A. [2 ]
El-Maraghy, Shohda A. [1 ]
Aziz, Wessam M. [2 ]
机构：
[1] Cairo Univ, Fac Pharm, Biochem Dept, Cairo, Egypt
[2] Natl Res Ctr, Therapeut Chem Dept, 33 El Bohouth St, Cairo, Egypt
来源：
JOURNAL OF BIOMEDICAL RESEARCH | 2019年 / 33卷 / 02期
关键词：
diabetic nephropathy; melatonin; rowatinex; histopathology; BETA-CELL DAMAGE; OXIDATIVE STRESS; EXPRESSION; KIDNEY; LIVER;
D O I：
10.7555/JBR.31.20160028
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
The study aimed to explore the prophylactic effect of melatonin, rowatinex; a naturally occurring renal drug, and its combination on diabetic nephropathy in type 2 diabetic rats. Diabetes was induced by intraperitoneal injection of a single dose of streptozotocin (50 mg/g body weight). Three days before diabetes induction, rats were daily treated with melatonin, rowatinex and their combination continuously for 8 weeks. Evaluation was done through measuring blood urea nitrogen (BUN), serum uric acid, serum creatinine, urine creatinine, creatinine clearance, nitric oxide (NO), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), total antioxidant capacity (TAC), kidney injury molecule-1 (KIM-1), heat shock protein-70 (HSP-70), caspase-3, transforming growth factor beta 1 (TGF beta 1), DNA degradation by the comet assay and total protein contents. Histopathologic study was also done for the kidney and the pancreas. Drastic changes in all measured parameters of the diabetic rats were observed. Treatment with melatonin and rowatinex showed amelioration to variable degrees. In conclusion, melatonin showed the most potent effect on protecting rats from deleterious action of diabetic nephropathy followed by its combination with rowatinex.
引用
收藏
页码：113 / 121
页数：9
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