Tissue microarrays: A powerful tool for high-throughput analysis of clinical specimens - A review of the method with validation data

被引:108
作者
Skacel, M [1 ]
Skilton, B [1 ]
Pettay, JD [1 ]
Tubbs, RR [1 ]
机构
[1] Cleveland Clin Fdn, Dept Anat Pathol, Cleveland, OH 44195 USA
来源
APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY | 2002年 / 10卷 / 01期
关键词
fluorescence in situ hybridization; immunohistochemistry; tissue microarray;
D O I
10.1097/00022744-200203000-00001
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
With recent advances in the knowledge of human molecular genetics, new gene-based disease mechanisms are emerging in many areas of medicine. The study of new prognostic and diagnostic markers in large numbers of clinical specimens is an important step in translating the new findings from basic science to clinical practice. The recently developed tissue microarray technology allows parallel molecular profiling of clinical samples at the DNA, RNA, and protein level. This technique enables pathologists to perform large-scale analyses using immunohistochemistry, fluorescence in situ hybridization, or RNA in situ hybridization substantially faster and at markedly lower costs compared with the conventional approach. This article provides a short review of this technology, focuses on several technical aspects of tissue microarray construction, and addresses the validity of the tissue microarray results for clinical research by reviewing data from recent literature along with the authors' own data.
引用
收藏
页码:1 / 6
页数:6
相关论文
共 14 条
  • [1] AFENYIANNAN A, 2001, MODERN PATHOL, V14, pA11
  • [2] BATTIFORA H, 1986, LAB INVEST, V55, P244
  • [3] BUBENDORF L, 1999, AM SOC HUM GEN 49 AN
  • [4] Validation of tissue microarray technology in breast carcinoma
    Camp, RL
    Charette, LA
    Rimm, DL
    [J]. LABORATORY INVESTIGATION, 2000, 80 (12) : 1943 - 1949
  • [5] Tissue microarrays for high-throughput molecular profiling of tumor specimens
    Kononen, J
    Bubendorf, L
    Kallioniemi, A
    Bärlund, M
    Schraml, P
    Leighton, S
    Torhorst, J
    Mihatsch, MJ
    Sauter, G
    Kallioniemi, OP
    [J]. NATURE MEDICINE, 1998, 4 (07) : 844 - 847
  • [6] High-throughput tissue microarray analysis to evaluate genes uncovered by cDNA microarray screening in renal cell carcinoma
    Moch, H
    Schraml, P
    Bubendorf, L
    Mirlacher, M
    Kononen, J
    Gasser, T
    Mihatsch, MJ
    Kallioniemi, OP
    Sauter, G
    [J]. AMERICAN JOURNAL OF PATHOLOGY, 1999, 154 (04) : 981 - 986
  • [7] Tissue microarrays: What will they bring to molecular and anatomic pathology?
    Moch, H
    Kononen, J
    Kallioniemi, OP
    Sauter, G
    [J]. ADVANCES IN ANATOMIC PATHOLOGY, 2001, 8 (01) : 14 - 20
  • [8] MOUSSES S, 2000, J UROLOGY, V163, pA101
  • [9] Neuroendocrine expression in metastatic prostate cancer: Evaluation of high throughput tissue microarrays to detect heterogeneous protein expression
    Mucci, NR
    Akdas, G
    Manely, S
    Rubin, MA
    [J]. HUMAN PATHOLOGY, 2000, 31 (04) : 406 - 414
  • [10] PROVERBSSINGH T, 2001, MODERN PATHOL, V14, pA117