Identification of Fusarium graminearum in cereal samples by DNA Detection Test Strips™

被引：26

作者：

Knoll, S ^{[1
]}

Vogel, RF ^{[1
]}

Niessen, L ^{[1
]}

机构：

[1] Tech Univ Munich, Lehrstuhl Tech Mikrobiol, D-85350 Freising Weihenstephan, Weihenstephan, Germany

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2002年 / 34卷 / 02期

关键词：

D O I：

10.1046/j.1472-765x.2002.01065.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aims: Development of a fast, sensitive and easy to handle method for the detection of Fusarium graminearum contamination in cereal samples by PCR. Methods and Results: DNA Detection Test Strips(TM) were used for PCR-product detection and the method was compared to agarose gel electrophoresis. A minimum of 0.26 ng of purified target DNA was detectable with the Test Strip(TM) Detection limit in less contaminated samples was slightly lower when gel electrophoresis was used for amplicon detection. In highly contaminated samples, detection limits of both methods were similar. Conclusions: Detection of PCR products was performed in 20 min without the need of special technical equipment or hazardous fluorescent DNA dyes. Significance and Impact of the Study: The new method described is useful for the screening of cereals in industrial quality control.

引用

页码：144 / 148

页数：5

共 23 条

[1]

BAI GH, 1994, PLANT DIS, V78, P760, DOI 10.1094/PD-78-0760

[2]

BUGLOVA T T, 1983, Mikrobiologicheskii Zhurnal (Kiev), V45, P47

[3] Fusarium molds and their mycotoxins [J].