SARS-CoV-2 viral load assessment in respiratory samples

被引：48

作者：

Kleiboeker, Steven ^{[1
]}

Cowden, Scott ^{[1
]}

Grantham, James ^{[1
]}

Nutt, Jamie ^{[1
]}

Tyler, Aaron ^{[1
]}

Berg, Amy ^{[1
]}

Altrich, Michelle ^{[1
]}

机构：

[1] Viracor Eurofins Clin Diagnost, Lees Summit, MO 64086 USA

来源：

JOURNAL OF CLINICAL VIROLOGY | 2020年 / 129卷

关键词：

SARS-CoV-2; Viral load diagnostic; RT-PCR;

D O I：

10.1016/j.jcv.2020.104439

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Real-time reverse transcriptase polymerase chain reaction (rRTPCR) has been the main method for diagnosis of SARS-CoV-2 infection in the early stages of the COVID-19 pandemic. De-identified results from upper and lower respiratory samples submitted to a reference laboratory demonstrated a positivity rate of 14.9 % (4428 of 29,713 samples tested). Distribution of results by birth year cohort and specimen type suggested general consistency in mean, median and peak values but higher positivity rates in individuals born from 1964 to 1974. Female patients had a significantly lower positivity rate (P < 0.0001), although similar load mean and median values, compared to males. Overall, 15.3 % (676 of 4428 positive results) of positive results had viral loads greater than 8 log10 copies/mL, with occasional samples exceeding 10 log10 copies/mL. These results support quantitative assessment of SARS-CoV-2 viral load in patient testing and efforts to control viral transmission

引用

页数：3

共 10 条

[1] Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR (Publication with Expression of Concern) [J].

Corman, Victor M. ;

Landt, Olfert ;

Kaiser, Marco ;

Molenkamp, Richard ;

Meijer, Adam ;

Chu, Daniel K. W. ;

Bleicker, Tobias ;

Bruenink, Sebastian ;

Schneider, Julia ;

Schmidt, Marie Luisa ;

Mulders, Daphne G. J. C. ;

Haagmans, Bart L. ;

van der Veer, Bas ;

van den Brink, Sharon ;

Wijsman, Lisa ;

Goderski, Gabriel ;

Romette, Jean-Louis ;

Ellis, Joanna ;

Zambon, Maria ;

Peiris, Malik ;

Goossens, Herman ;

Reusken, Chantal ;

Koopmans, Marion P. G. ;

Drosten, Christian .

EUROSURVEILLANCE, 2020, 25 (03) :23-30

[2] Long-Term Outcomes in Patients Undergoing Percutaneous Coronary Intervention with or without Preprocedural Exercise Stress Test [J].

Kim, Jihoon ;

Lee, Joo Myung ;

Park, Taek Kyu ;

Yang, Jeong Hoon ;

Song, Young Bin ;

Choi, Jin-Ho ;

Choi, Seung-Hyuk ;

Gwon, Hyeon-Cheol ;

Lee, Sang Hoon ;

Hahn, Joo-Yong .

JOURNAL OF KOREAN MEDICAL SCIENCE, 2020, 35 (01)

[3] A novel multiplex real-time RT-PCR assay with FRET hybridization probes for the detection and quantitation of 13 respiratory viruses [J].

Lassauniere, R. ;

Kresfelder, T. ;

Venter, M. .

JOURNAL OF VIROLOGICAL METHODS, 2010, 165 (02) :254-260

[4] Viral Load Kinetics of MERS Coronavirus Infection [J].

Oh, Myoung-don ;

Park, Wan Beom ;

Choe, Pyoeng Gyun ;

Choi, Su-Jin ;

Kim, Jong-Il ;

Chae, Jeesoo ;

Park, Sung Sup ;

Kim, Eui-Chong ;

Oh, Hong Sang ;

Kim, Eun Jung ;

Nam, Eun Young ;

Na, Sun Hee ;

Kim, Dong Ki ;

Lee, Sang-Min ;

Song, Kyoung-Ho ;

Bang, Ji Hwan ;

Kim, Eu Suk ;

Kim, Hong Bin ;

Park, Sang Won ;

Kim, Nam Joong .

NEW ENGLAND JOURNAL OF MEDICINE, 2016, 375 (13) :1303-1305

[5] Viral load of SARS-CoV-2 in clinical samples [J].

Pan, Yang ;

Zhang, Daitao ;

Yang, Peng ;

Poon, Leo L. M. ;

Wang, Quanyi .

LANCET INFECTIOUS DISEASES, 2020, 20 (04) :411-412

[6] Clinical progression and viral load in a community outbreak of coronavirus-associated SARS pneumonia: a prospective study [J].

Peiris, JSM ;

Chu, CM ;

Cheng, VCC ;

Chan, KS ;

Hung, IFN ;

Poon, LLM ;

Law, KI ;

Tang, BSF ;

Hon, TYW ;

Chan, CS ;

Chan, KH ;

Ng, JSC ;

Zheng, BJ ;

Ng, WL ;

Lai, RWM ;

Guan, Y ;

Yuen, KY .

LANCET, 2003, 361 (9371) :1767-1772

[7] Temporal profiles of viral load in posterior oropharyngeal saliva samples and serum antibody responses during infection by SARS-CoV-2: an observational cohort study [J].

To, Kelvin Kai-Wang ;

Tsang, Owen Tak-Yin ;

Leung, Wai-Shing ;

Tam, Anthony Raymond ;

Wu, Tak-Chiu ;

Lung, David Christopher ;

Yip, Cyril Chik-Yan ;

Cai, Jian-Piao ;

Chan, Jacky Man-Chun ;

Chik, Thomas Shiu-Hong ;

Lau, Daphne Pui-Ling ;

Choi, Chris Yau-Chung ;

Chen, Lin-Lei ;

Chan, Wan-Mui ;

Chan, Kwok-Hung ;

Ip, Jonathan Daniel ;

Ng, Anthony Chin-Ki ;

Poon, Rosana Wing-Shan ;

Luo, Cui-Ting ;

Cheng, Vincent Chi-Chung ;

Chan, Jasper F. W. ;

Hung, Ivan Fan-Ngai ;

Chen, Zhiwei ;

Chen, Honglin ;

Yuen, Kwok-Yung .

LANCET INFECTIOUS DISEASES, 2020, 20 (05) :565-574

[8] Detection of SARS-CoV-2 in Different Types of Clinical Specimens [J].

Wang, Wenling ;

Xu, Yanli ;

Gao, Ruqin ;

Lu, Roujian ;

Han, Kai ;

Wu, Guizhen ;

Tan, Wenjie .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2020, 323 (18) :1843-1844

[9] Virological assessment of hospitalized patients with COVID-2019 [J].

Woelfel, Roman ;

Corman, Victor M. ;

Guggemos, Wolfgang ;

Seilmaier, Michael ;

Zange, Sabine ;

Mueller, Marcel A. ;

Niemeyer, Daniela ;

Jones, Terry C. ;

Vollmar, Patrick ;

Rothe, Camilla ;

Hoelscher, Michael ;

Bleicker, Tobias ;

Bruenink, Sebastian ;

Schneider, Julia ;

Ehmann, Rosina ;

Zwirglmaier, Katrin ;

Drosten, Christian ;

Wendtner, Clemens .

NATURE, 2020, 581 (7809) :465-+

[10]

Zou LR, 2020, NEW ENGL J MED, V382, P1177, DOI [10.1148/radiol.2020200463, 10.1056/NEJMc2001737]

← 1 →