Cell surface proteomics identifies glucose transporter type 1 and prion protein as candidate biomarkers for colorectal adenoma-to-carcinoma progression
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de Wit, Meike
[1
,2
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Jimenez, Connie R.
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Vrije Univ Amsterdam Med Ctr, Dept Med Oncol, OncoProte Lab, NL-1081 HV Amsterdam, NetherlandsVrije Univ Amsterdam Med Ctr, Dept Pathol, Tumor Profiling Unit, NL-1081 HV Amsterdam, Netherlands
Jimenez, Connie R.
[2
]
Carvalho, Beatriz
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Vrije Univ Amsterdam Med Ctr, Dept Pathol, Tumor Profiling Unit, NL-1081 HV Amsterdam, NetherlandsVrije Univ Amsterdam Med Ctr, Dept Pathol, Tumor Profiling Unit, NL-1081 HV Amsterdam, Netherlands
Carvalho, Beatriz
[1
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Belien, Jeroen A. M.
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Vrije Univ Amsterdam Med Ctr, Dept Pathol, Tumor Profiling Unit, NL-1081 HV Amsterdam, NetherlandsVrije Univ Amsterdam Med Ctr, Dept Pathol, Tumor Profiling Unit, NL-1081 HV Amsterdam, Netherlands
Belien, Jeroen A. M.
[1
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Delis-van Diemen, Pien M.
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Vrije Univ Amsterdam Med Ctr, Dept Pathol, Tumor Profiling Unit, NL-1081 HV Amsterdam, NetherlandsVrije Univ Amsterdam Med Ctr, Dept Pathol, Tumor Profiling Unit, NL-1081 HV Amsterdam, Netherlands
Delis-van Diemen, Pien M.
[1
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Mongera, Sandra
[1
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Piersma, Sander R.
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Vrije Univ Amsterdam Med Ctr, Dept Med Oncol, OncoProte Lab, NL-1081 HV Amsterdam, NetherlandsVrije Univ Amsterdam Med Ctr, Dept Pathol, Tumor Profiling Unit, NL-1081 HV Amsterdam, Netherlands
Piersma, Sander R.
[2
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Vikas, Maindad
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Lab Surg Path, Bombay, Maharashtra, IndiaVrije Univ Amsterdam Med Ctr, Dept Pathol, Tumor Profiling Unit, NL-1081 HV Amsterdam, Netherlands
Vikas, Maindad
[3
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Navani, Sanjay
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Lab Surg Path, Bombay, Maharashtra, IndiaVrije Univ Amsterdam Med Ctr, Dept Pathol, Tumor Profiling Unit, NL-1081 HV Amsterdam, Netherlands
Navani, Sanjay
[3
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Ponten, Fredrik
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Uppsala Univ, Rudbeck Lab, Dept Genet & Pathol, Uppsala, SwedenVrije Univ Amsterdam Med Ctr, Dept Pathol, Tumor Profiling Unit, NL-1081 HV Amsterdam, Netherlands
Ponten, Fredrik
[4
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Meijer, Gerrit A.
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Vrije Univ Amsterdam Med Ctr, Dept Pathol, Tumor Profiling Unit, NL-1081 HV Amsterdam, NetherlandsVrije Univ Amsterdam Med Ctr, Dept Pathol, Tumor Profiling Unit, NL-1081 HV Amsterdam, Netherlands
Meijer, Gerrit A.
[1
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Fijneman, Remond J. A.
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Vrije Univ Amsterdam Med Ctr, Dept Pathol, Tumor Profiling Unit, NL-1081 HV Amsterdam, NetherlandsVrije Univ Amsterdam Med Ctr, Dept Pathol, Tumor Profiling Unit, NL-1081 HV Amsterdam, Netherlands
Fijneman, Remond J. A.
[1
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机构:
[1] Vrije Univ Amsterdam Med Ctr, Dept Pathol, Tumor Profiling Unit, NL-1081 HV Amsterdam, Netherlands
[2] Vrije Univ Amsterdam Med Ctr, Dept Med Oncol, OncoProte Lab, NL-1081 HV Amsterdam, Netherlands
[3] Lab Surg Path, Bombay, Maharashtra, India
[4] Uppsala Univ, Rudbeck Lab, Dept Genet & Pathol, Uppsala, Sweden
Background and objective Early detection of colon adenomas at high risk of progression and early-stage colorectal cancer (CRC) is an effective approach to reduce CRC death rates. Current screening methods lack specificity as they detect many adenomas that will never progress to CRC. The authors aimed to identify cell surface protein biomarkers with extracellular domains that could be targeted for molecular imaging and discriminate low-risk adenomas and normal colon from high-risk adenomas and CRC. Design Cell surface proteins of five CRC cell lines were biotinylated, isolated and analysed by in-depth proteomics using gel electrophoresis and nanoliquid chromatography coupled to tandem mass spectrometry. Differential expression in adenomas and CRCs was based on mRNA expression and verified by immunohistochemical staining of tissue microarrays. Results In total, 2609 proteins were identified in the cell surface fractions. Of these, 44 proteins were selected as promising cell surface candidate biomarkers for adenoma-to-carcinoma progression based on the following criteria: protein identification in at least four out of five cell lines, a predicted (trans) membrane location and increased mRNA expression in CRCs compared to adenomas. Increased protein expression in high-risk adenomas and CRCs compared to low-risk adenomas was confirmed by immunohistochemistry for glucose transporter type 1 (gene symbol SLC2A1; p<0.00001) and prion protein (gene symbol PRNP; p<0.005). Conclusion This study revealed glucose transporter type 1, prion protein and 42 other cell surface candidate biomarkers for adenoma-to-carcinoma progression that could potentially serve as targets for emerging molecular imaging modalities like optical imaging, F-19-MRI and positron emission tomography.
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Ji Nan Univ, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China
Ji Nan Univ, Chinese Univ Hong Kong, Joint Lab Regenrat Med, Guangzhou, Guangdong, Peoples R ChinaJi Nan Univ, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China
Cai, Dongqing
Cao, Jie
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Guangzhou Med Univ, Affiliated Guangzhou Peoples Hosp 1, Dept Surg, Guangzhou, Guangdong, Peoples R ChinaJi Nan Univ, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China
Cao, Jie
Li, Zhen
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Ji Nan Univ, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China
Ji Nan Univ, Chinese Univ Hong Kong, Joint Lab Regenrat Med, Guangzhou, Guangdong, Peoples R ChinaJi Nan Univ, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China
Li, Zhen
Zheng, Xin
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Ji Nan Univ, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China
Ji Nan Univ, Chinese Univ Hong Kong, Joint Lab Regenrat Med, Guangzhou, Guangdong, Peoples R ChinaJi Nan Univ, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China
Zheng, Xin
Yao, Yao
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Ji Nan Univ, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China
Ji Nan Univ, Chinese Univ Hong Kong, Joint Lab Regenrat Med, Guangzhou, Guangdong, Peoples R ChinaJi Nan Univ, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China
Yao, Yao
Li, Wanglin
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Guangzhou Med Univ, Affiliated Guangzhou Peoples Hosp 1, Dept Surg, Guangzhou, Guangdong, Peoples R ChinaJi Nan Univ, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China
Li, Wanglin
Yuan, Ziqiang
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Ji Nan Univ, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China
Ji Nan Univ, Chinese Univ Hong Kong, Joint Lab Regenrat Med, Guangzhou, Guangdong, Peoples R China
Albert Einstein Coll Med, Dept Mol Genet, New York, NY USAJi Nan Univ, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China
机构:
Ji Nan Univ, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China
Ji Nan Univ, Chinese Univ Hong Kong, Joint Lab Regenrat Med, Guangzhou, Guangdong, Peoples R ChinaJi Nan Univ, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China
Cai, Dongqing
Cao, Jie
论文数: 0引用数: 0
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Guangzhou Med Univ, Affiliated Guangzhou Peoples Hosp 1, Dept Surg, Guangzhou, Guangdong, Peoples R ChinaJi Nan Univ, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China
Cao, Jie
Li, Zhen
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Ji Nan Univ, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China
Ji Nan Univ, Chinese Univ Hong Kong, Joint Lab Regenrat Med, Guangzhou, Guangdong, Peoples R ChinaJi Nan Univ, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China
Li, Zhen
Zheng, Xin
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Ji Nan Univ, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China
Ji Nan Univ, Chinese Univ Hong Kong, Joint Lab Regenrat Med, Guangzhou, Guangdong, Peoples R ChinaJi Nan Univ, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China
Zheng, Xin
Yao, Yao
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Ji Nan Univ, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China
Ji Nan Univ, Chinese Univ Hong Kong, Joint Lab Regenrat Med, Guangzhou, Guangdong, Peoples R ChinaJi Nan Univ, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China
Yao, Yao
Li, Wanglin
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Guangzhou Med Univ, Affiliated Guangzhou Peoples Hosp 1, Dept Surg, Guangzhou, Guangdong, Peoples R ChinaJi Nan Univ, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China
Li, Wanglin
Yuan, Ziqiang
论文数: 0引用数: 0
h-index: 0
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Ji Nan Univ, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China
Ji Nan Univ, Chinese Univ Hong Kong, Joint Lab Regenrat Med, Guangzhou, Guangdong, Peoples R China
Albert Einstein Coll Med, Dept Mol Genet, New York, NY USAJi Nan Univ, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China