Establishment and characterization of a novel 'double-hit' follicular lymphoma cell line, FL-SJC

被引：0

作者：

Chen, Min ^{[1
]}

Jiang, Guoxiong ^{[1
,2
]}

Liu, Yichen ^{[1
]}

Li, Dongya ^{[1
]}

Li, Tiantian ^{[1
]}

Peng, Jie ^{[1
]}

Jiang, Qian ^{[1
]}

You, Haiyan ^{[1
]}

Ba, Rong ^{[1
]}

Pan, Jinlan ^{[3
]}

Li, Mei ^{[1
]}

Long, Weiguo ^{[1
]}

Yan, Jinsong ^{[5
]}

Zhu, Yan ^{[1
]}

Wang, Yun ^{[4
]}

Xi, Xiaodong ^{[4
]}

Mao, Jianhua ^{[4
]}

Shi, Xiaofeng ^{[1
,6
]}

机构：

[1] Jiangsu Univ, Affiliated Hosp, 438 North Jiefang Rd, Zhenjiang, Jiangsu, Peoples R China

[2] Jiangdu Peoples Hosp, Yangzhou, Jiangsu, Peoples R China

[3] Suzhou Univ, Affiliated Hosp, Suzhou, Peoples R China

[4] Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, State Key Lab Med Genom,Shanghai Inst Hematol, 197 Second Ruijin Rd, Shanghai, Peoples R China

[5] Dalian Med Univ, Hosp 2, Dalian, Peoples R China

[6] Nanjing Med Univ, Affiliated Hosp 2, Nanjing, Peoples R China

来源：

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | 2020年 / 24卷 / 14期

基金：

中国国家自然科学基金;

关键词：

BCL2; Double-hit lymphoma; FL-SJC cell line; Follicular lymphoma; MYC; IN-VITRO; GUIDELINES; GENES; FL-18;

D O I：

10.1111/jcmm.15425

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

About 5 per cent of follicular lymphoma (FL) cases are double-hit (DH) lymphomas. Double-hit follicular lymphoma (DHFL) cell lines can improve our understanding and drug development on FL. But there are only few DHFL cell lines. Here, we established a new MYC/BCL2 DHFL cell line, FL-SJC. The cells were obtained from the hydrothorax of a patient with MYC/BCL2 DHFL and cultured for 140 passages in vitro. FL-SJC cells demonstrated CD19(++), CD20(+), CD22(++), HLA-DR+, CD10(+), CD38(+), Lambda(+) CD23(-), CD5(-) and Kappa(-). The chromosome karyotypic analysis confirmed the co-existence of t(8;22)(q24;q11) and t(14;18)(q32;q21), as well as additional abnormalities involving chromosomes 2 and 3. Fluorescence in situ hybridization analysis (FISH) showed IGH/BCL2 fusion gene and the MYC rearrangement. In addition, the FL-SJC cells displayed KMT2D/MLL2 and CREBBP gene mutations. After subcutaneous inoculation of FL-SJC cells, the SCID mice developed solid tumour masses within 6-8 weeks. FL-SJC cells were proven to be free of Epstein-Barr (EB) virus infection and be multidrug-resistant. In a conclusion, the FL-SJC cell line has been identified as a novel MYC/BCL2 double-hit follicular lymphoma that can be used as a potentially available tool for the clinical and basic research, together with the drug development for MYC/BCL2 DHFL.

引用

页码：7928 / 7938

页数：11

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