Affinity Sorbent Based on Tryptophyl-Threonyl-Tyrosine for Binding of the Immunoglobulins G: Sorption Characteristics and Aspects of Practical Application

被引：6

作者：

Levashov, P. A. ^{[1
]}

Ovchinnikova, E. D. ^{[1
]}

Afanas'eva, M. I. ^{[1
]}

Frid, D. A. ^{[1
]}

Az'muko, A. A. ^{[1
]}

Bespalova, Zh. D. ^{[1
]}

Adamova, I. Yu. ^{[1
]}

Afanas'eva, O. I. ^{[1
]}

Pokrovskii, S. N. ^{[1
]}

机构：

[1] Minist Hlth & Social Dev Russian Federat, Fed State Inst Russian Cardiol Res & Prod Complex, Moscow 121552, Russia

来源：

RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2012年 / 38卷 / 01期

关键词：

affinity sorbent; tripeptide; tryptophyl-threonyl-tyrosine as a ligand; immunoglobulins; affinity chromatography; SYSTEMIC-LUPUS-ERYTHEMATOSUS; PROTEIN-A; HUMAN-IGG; IMMUNOADSORPTION; APHERESIS; MOLECULE; LIGANDS; DESIGN;

D O I：

10.1134/S1068162012010141

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chromatographic material based on tryptophil-threonyl-tyrosine and efficiently binding human, sheep, goat, and bovine immunoglobulin G was obtained. High selectivity of the sorbent for extraction of immunoglobulins from blood plasma has been demonstrated. Effective sorption capacity is 15-25 mg of immunoglobulin G per 1 ml of the matrix. Optimization of the method of triptophyl-threonyl-tyrosine covalent binding to the polysaccharide matrix allowed the achievement of high sorbent stability in conditions of use and storage. This sorbent may be used in medicine and biotechnology.

引用

页码：46 / 50

页数：5

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