Live Imaging of Bicoid-Dependent Transcription in Drosophila Embryos

被引:114
作者
Lucas, Tanguy [1 ,2 ,4 ]
Ferraro, Teresa [1 ,2 ,3 ,4 ]
Roelens, Baptiste [1 ,2 ,4 ]
Chanes, Jose De Las Heras [1 ,2 ]
Walczak, Aleksandra M. [2 ,3 ,4 ]
Coppey, Mathieu [1 ,2 ,3 ,4 ]
Dostatni, Nathalie [1 ,2 ,4 ]
机构
[1] Inst Curie, Ctr Rech, F-75248 Paris, France
[2] CNRS, UMR218, UMR168, UMR8549,UMR8550, F-75248 Paris, France
[3] ENS, UMR8549, UMR8550, F-75005 Paris, France
[4] UPMC, F-75248 Paris, France
关键词
POSITIONAL INFORMATION; GENE-EXPRESSION; GRADIENT; HUNCHBACK; LOCALIZATION; PRECISION; DYNAMICS; PATTERNS; TIME;
D O I
10.1016/j.cub.2013.08.053
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The early Drosophila embryo is an ideal model to understand the transcriptional regulation of well-defined patterns of gene expression in a developing organism [1]. In this system, snapshots of transcription measurements obtained by RNA FISH on fixed samples [2,3] cannot provide the temporal resolution needed to distinguish spatial heterogeneity [3] from inherent noise [4, 5]. Here, we used the MS2-MCP system [6, 7] to visualize in living embryos nascent transcripts expressed from the canonical hunchback (hb) promoter [8, 9] under the control of Bicoid (Bcd) [10]. The hb-MS2 reporter is expressed as synchronously as endogenous hb in the anterior half of the embryo, but unlike hb it is also active in the posterior, though more heterogeneously and more transiently than in the anterior. The length and intensity of active transcription periods in the anterior are strongly reduced in absence of Bcd, whereas posterior ones are mostly Bcd independent. This posterior noisy signal decreases progressively through nuclear divisions, so that the MS2 reporter expression mimics the known anterior hb pattern at cellularization. We propose that the establishment of the hb pattern relies on Bcd-dependent lengthening of transcriptional activity periods in the anterior and may require two distinct repression mechanisms in the posterior.
引用
收藏
页码:2135 / 2139
页数:5
相关论文
共 19 条
  • [1] Localization of ASH1 mRNA particles in living yeast
    Bertrand, E
    Chartrand, P
    Schaefer, M
    Shenoy, SM
    Singer, RH
    Long, RM
    [J]. MOLECULAR CELL, 1998, 2 (04) : 437 - 445
  • [2] Synchronous and Stochastic Patterns of Gene Activation in the Drosophila Embryo
    Boettiger, Alistair N.
    Levine, Michael
    [J]. SCIENCE, 2009, 325 (5939) : 471 - 473
  • [3] THE BICOID PROTEIN IS A POSITIVE REGULATOR OF HUNCHBACK TRANSCRIPTION IN THE EARLY DROSOPHILA EMBRYO
    DRIEVER, W
    NUSSLEINVOLHARD, C
    [J]. NATURE, 1989, 337 (6203) : 138 - 143
  • [4] Measurement and Perturbation of Morphogen Lifetime: Effects on Gradient Shape
    Drocco, Jeffrey A.
    Grimm, Oliver
    Tank, David W.
    Wieschaus, Eric
    [J]. BIOPHYSICAL JOURNAL, 2011, 101 (08) : 1807 - 1815
  • [5] Functional roles for noise in genetic circuits
    Eldar, Avigdor
    Elowitz, Michael B.
    [J]. NATURE, 2010, 467 (7312) : 167 - 173
  • [6] Live imaging of endogenous RNA reveals a diffusion and entrapment mechanism for nanos mRNA localization in Drosophila
    Forrest, KM
    Gavis, ER
    [J]. CURRENT BIOLOGY, 2003, 13 (14) : 1159 - 1168
  • [7] Probing the limits to positional information
    Gregor, Thomas
    Tank, David W.
    Wieschaus, Eric F.
    Bialek, William
    [J]. CELL, 2007, 130 (01) : 153 - 164
  • [8] Stability and nuclear dynamics of the bicoid morphogen gradient
    Gregor, Thomas
    Wieschaus, Eric F.
    McGregor, Alistair P.
    Bialek, William
    Tank, David W.
    [J]. CELL, 2007, 130 (01) : 141 - 152
  • [9] Modelling the Bicoid gradient
    Grimm, Oliver
    Coppey, Mathieu
    Wieschaus, Eric
    [J]. DEVELOPMENT, 2010, 137 (14): : 2253 - 2264
  • [10] Drosophila blastoderm patterning
    Jaeger, Johannes
    Manu
    Reinitz, John
    [J]. CURRENT OPINION IN GENETICS & DEVELOPMENT, 2012, 22 (06) : 533 - 541