Determination of glutathione and related aminothiols in mouse tissues by gas chromatography with flame photometric detection

被引：9
|
作者：
Takagi, K [1 ]
Kataoka, H [1 ]
Makita, M [1 ]
机构：
[1] OKAYAMA UNIV, FAC PHARMACEUT SCI, OKAYAMA 700, JAPAN
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1996年 / 60卷 / 04期
关键词：
N; S-isopropoxycarbonyl methyl ester derivatives; gas chromatography; flame photometric detection; redox status;
D O I：
10.1271/bbb.60.729
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A selective and sensitive method for the determination of glutathione and such related aminothiols as Cys, cysteinylglycine and gamma-glutamylcysteine in mouse tissues by gas chromatography was developed. After fractionating a tissue sample into the free and protein-bound fractions, glutathione and aminothiols in each fraction were converted into their N,S-isopropoxycarbonyl methyl ester derivatives and then measured by gas chromatography with flame photometric detection, using a DB-1 capillary column. This method enabled the redox status of glutathione and related aminothiols in mouse tissues to be investigated.
引用
收藏
页码：729 / 731
页数：3
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