Rhodamine-based 'turn-on' fluorescent probe for Cu(II) and its fluorescence imaging in living cells

被引：46
作者：
Tian, Mao-Zhong [1 ,2 ]
Hu, Ming-Ming [1 ]
Fan, Jiang-Li [1 ]
Peng, Xiao-Jun [1 ]
Wang, Jing-Yun [1 ]
Sun, Shi-Guo [1 ]
Zhang, Rong [1 ]
机构：
[1] Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China
[2] Shanxi Datong Univ, Coll Chem & Chem Engn, Datong 037009, Peoples R China
来源：
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2013年 / 23卷 / 10期
关键词：
Fluorescence; Probe; Cu2+; Rhodamine; Cell imaging; NANOMOLAR DETECTION; AQUEOUS-SOLUTION; SENSOR; CU2+; COPPER(II); CHEMOSENSOR; CHEMODOSIMETERS; SENSITIVITY; SELECTIVITY; WATER;
D O I：
10.1016/j.bmcl.2013.03.052
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
A novel rhodamine spirolactam derivative 3',6'-Bis(diethylamino)-2-(2-hydroxyethylamino) spiro[iso-indoline-1,9'-xanthen]-3-one (RO1) was synthesized, and characterized by high-resolution mass spectrometry (HRMS), X-ray crystallography, Infrared spectroscopy (IR), and H-1 NMR and C-13 NMR spectroscopy. RO1 exhibited highly sensitive and exclusively selective fluorescence response toward Cu2+ over other metal ions with a detection limit of 0.56 ppb in mixed aqueous solution. The fluorescence was pH-independent in the wide range pH 3.1-11.6. The turn-on fluorescence enhancement of the probe is based on Cu2+ induced ring-opening mechanism of the rhodamine spirolactam. Moreover, by means of fluorescence microscopy experiments, it was demonstrated that RO1 could monitor trace Cu2+ changes by live cell imaging. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码：2916 / 2919
页数：4
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