Thermostable Branched DNA Nanostructures as Modular Primers for Polymerase Chain Reaction

被引：77

作者：

Hartman, Mark R. ^{[1
]}

Yang, Dayong ^{[1
]}

Tran, Thua N. N. ^{[1
]}

Lee, Kwang ^{[1
]}

Kahn, Jason S. ^{[1
]}

Kiatwuthinon, Pichamon ^{[1
]}

Yancey, Kenneth G. ^{[1
]}

Trotsenko, Oleksandr ^{[3
]}

Minko, Sergiy ^{[3
]}

Luo, Dan ^{[1
,2
]}

机构：

[1] Cornell Univ, Dept Biol & Environm Engn, Ithaca, NY 14853 USA

[2] Cornell Univ, Kavli Inst Cornell Nanoscale Sci, Ithaca, NY 14853 USA

[3] Clarkson Univ, Dept Chem & Biomol Sci, Potsdam, NY 13699 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 33期

基金：

比尔及梅琳达.盖茨基金会;

关键词：

DNA structures; hydrogels; nanotechnology; nucleic acids; polymerase chain reaction; SINGLE-MOLECULE; SHAPES;

D O I：

10.1002/anie.201302175

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Chemical cross-linking was used to prepare DNA nanostructures with enhanced thermal stability. These thermostable DNA nanostructures were then utilized as modular primers in the polymerase chain reaction (PCR; see picture), thus enabling the production of multifunctionalized and branched PCR products for multiplexed detection and hydrogel formation. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：8699 / 8702

页数：4

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