Lipoxygenases: Structural principles and spectroscopy

被引：81
作者：
Gaffney, BJ
机构：
[1] Chemistry Department, Johns Hopkins University, Baltimore
来源：
ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE | 1996年 / 25卷
关键词：
lipoxygenase; X-ray structure; pi-helix; non-heme iron; metalloprotein; c-terminus; EMR; EPR; XAS; NIR CD; MCD;
D O I：
10.1146/annurev.bb.25.060196.002243
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Lipoxygenases catalyze the formation of fatty acid hydroperoxides, products used in further biochemical reactions leading to normal and pathological cell functions. X-ray structure analysis and spectroscopy have been applied to elucidate the mechanism of lipoxygenases. Two X-ray structures of soybean lipoxygenase-1 reveal the side chains of three histidines and the COO- of the carboxy terminus as ligands to the catalytically important iron atom. The enzyme contains a novel three-turn pi-helix near the iron center. Spectroscopic studies, including electron magnetic resonance, X-ray absorption spectroscopy, infrared circular dichroism, and magnetic circular dichroism, have been applied to compare lipoxygenases from varied sources and with different substrate positional specificity.
引用
收藏
页码：431 / 459
页数：29
相关论文
共 100 条
[81]   THE MOLECULAR-BIOLOGY OF MAMMALIAN ARACHIDONIC-ACID METABOLISM [J].
SIGAL, E .
AMERICAN JOURNAL OF PHYSIOLOGY, 1991, 260 (02) :L13-L28
[82]   A QUANTITATIVE OPTICAL AND ELECTRON-PARAMAGNETIC-RES STUDY ON THE INTERACTION BETWEEN SOYBEAN LIPOXYGENASE-1 AND 13-L-HYDROPEROXYLINOLEIC ACID [J].
SLAPPENDEL, S ;
VELDINK, GA ;
VLIEGENTHART, JFG ;
AASA, R ;
MALMSTROM, BG .
BIOCHIMICA ET BIOPHYSICA ACTA, 1983, 747 (1-2) :32-36
[83]   FACTORS AFFECTING THE LINE-SHAPE OF THE ELECTRON-PARAMAGNETIC-RES SIGNAL OF HIGH-SPIN FE(III) IN SOYBEAN LIPOXYGENASE-1 [J].
SLAPPENDEL, S ;
AASA, R ;
MALMSTROM, BG ;
VERHAGEN, J ;
VELDINK, GA ;
VLIEGENTHART, JFG .
BIOCHIMICA ET BIOPHYSICA ACTA, 1982, 708 (03) :259-265
[84]   ELECTRON-PARAMAGNETIC-RES SPECTROSCOPY OF SOYBEAN LIPOXYGENASE-1 - DESCRIPTION AND QUANTIFICATION OF THE HIGH-SPIN FE(III) SIGNALS [J].
SLAPPENDEL, S ;
VELDINK, GA ;
VLIEGENTHART, JFG ;
AASA, R ;
MALMSTROM, BG .
BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 667 (01) :77-86
[85]   PURIFICATION AND CRYSTALLIZATION OF 15-LIPOXYGENASE FROM RABBIT RETICULOCYTES [J].
SLOANE, DL ;
BROWNER, MF ;
DAUTER, Z ;
WILSON, K ;
FLETTERICK, RJ ;
SIGAL, E .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 173 (02) :507-513
[86]   A PRIMARY DETERMINANT FOR LIPOXYGENASE POSITIONAL SPECIFICITY [J].
SLOANE, DL ;
LEUNG, R ;
CRAIK, CS ;
SIGAL, E .
NATURE, 1991, 354 (6349) :149-152
[87]   THE ELECTRONIC-STRUCTURES OF ACTIVE-SITES IN NONHEME IRON ENZYMES [J].
SOLOMON, EI ;
YAN, Z .
ACCOUNTS OF CHEMICAL RESEARCH, 1992, 25 (08) :343-352
[88]   CRYSTALLIZATION AND PRELIMINARY-X-RAY CHARACTERIZATION OF A SOYBEAN SEED LIPOXYGENASE [J].
STALLINGS, WC ;
KROA, BA ;
CARROLL, RT ;
METZGER, AL ;
FUNK, MO .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 211 (04) :685-687
[89]   CONSERVED HISTIDINE-RESIDUES IN SOYBEAN LIPOXYGENASE - FUNCTIONAL CONSEQUENCES OF THEIR REPLACEMENT [J].
STECZKO, J ;
DONOHO, GP ;
CLEMENS, JC ;
DIXON, JE ;
AXELROD, B .
BIOCHEMISTRY, 1992, 31 (16) :4053-4057
[90]   THE 2.1-A RESOLUTION STRUCTURE OF IRON SUPEROXIDE-DISMUTASE FROM PSEUDOMONAS-OVALIS [J].
STODDARD, BL ;
HOWELL, PL ;
RINGE, D ;
PETSKO, GA .
BIOCHEMISTRY, 1990, 29 (38) :8885-8893
12345678910