Imaging of metals and metal-containing species in biological tissues and on gels by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS): A new analytical strategy for applications in life sciences

被引:28
作者
Becker, J. Sabine [1 ]
Zoriy, M. [1 ]
Dressler, Valderi L. [3 ]
Wu, Bei [1 ,4 ]
Becker, J. Susanne [2 ]
机构
[1] Res Ctr Julich, Cent Div Analyt Chem, D-52425 Julich, Germany
[2] CNRS, UMR 5034, Grp Bioinorgan Analyt Chem, F-64053 Pau, France
[3] Univ Fed Santa Maria, Dept Chem, BR-97105900 Santa Maria, RS, Brazil
[4] Zhejiang Univ, Inst Environm Sci & Technol, Hangzhou 310029, Zhejiang, Peoples R China
关键词
biological tissues; imaging mass spectrometry; LA-ICP-MS; metal distribution; element distribution;
D O I
10.1351/pac200880122643
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has become. established as a very efficient and sensitive trace, ultratrace, and surface analytical technique in the life sciences. We have developed a new analytical imaging technique using LA-ICP-MS to study element distribution in biological tissues. Nowadays, imaging LA-ICP-MS using double-focusing sector field (LA-ICP-SFMS) or quadrupole-based mass spectrometers (LA-ICP-QMS) can be applied as an exciting tool providing new information on the pathophysiology, pharmacology, and toxicology of elements of interest in biological systems. The quantitative determination of elements (e.g., Cu, Fe, Zn, Se, and others) in biological tissues is of growing interest especially in brain research (e.g., for studying neurode-generative diseases such as Alzheimer's or Parkinson's disease). LA-ICP-SFMS was employed to produce images of detailed regionally specific element distributions in thin tissue sections of different sizes (such as control human or rat brain tissues or tumor regions). In addition, imaging MS using LA-ICP-QMS was applied to study the uptake and transport of nutrient and toxic elements in plant tissues. Besides the quantitative imaging of essential and toxic elements in tissues, powerful analytical techniques are also required for the determination and characterization of phosphoproteins and metal-containing proteins within a large pool of proteins, after electrophoretic separation [e.g., blue native polyacrylamide gel electrophoresis (BN-PAGE) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)] into ID and 2D gels. LA-ICP-MS was used to detect metalloproteins in protein bands of ID gels or protein spots separated after 2D gel electrophoresis (2D-GE). In addition to elemental determination by LA-ICP-MS, matrix-assisted laser desorption/ionization, or MALDI-MS, was employed to identify metal-containing proteins. Recent progress will be discussed in applying LA-ICP-MS in the life sciences, including the imaging of thin slices of tissue and applications in proteome analysis in combination with MALDI-MS to investigate phosphoproteins and metal-containing proteins.
引用
收藏
页码:2643 / 2655
页数:13
相关论文
共 31 条
[1]   Quantitative imaging of selenium, copper, and zinc in thin sections of biological tissues (Slugs-Genus arion) measured by laser ablation inductively coupled of plasma mass spectrometry [J].
Becker, J. S. ;
Matusch, A. ;
Depboylu, C. ;
Dobrowolska, J. ;
Zoriy, M. V. .
ANALYTICAL CHEMISTRY, 2007, 79 (16) :6074-6080
[2]  
Becker J. S., 2007, INORGANIC MASS SPECT
[3]   Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) in elemental imaging of biological tissues and in proteomicsw [J].
Becker, J. Sabine ;
Zoriy, Miroslav ;
Becker, J. Susanne ;
Dobrowolska, Justina ;
Matusch, Andreas .
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, 2007, 22 (07) :736-744
[4]   Study of formation of Cu- and Zn-containing tau protein using isotopically-enriched tracers by LA-ICP-MS and MALDI-FTICR-MS [J].
Becker, J. Susanne ;
Zoriy, Miroslav ;
Przybylski, Michael ;
Becker, J. Sabine .
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, 2007, 22 (01) :63-68
[5]   High resolution mass spectrometric brain proteomics by MALDI-FTICR-MS combined with determination of P, S, Cu, Zn and Fe by LA-ICP-MS [J].
Becker, J. Susanne ;
Zoriy, Miroslav ;
Przybylski, Michael ;
Becker, J. Sabine .
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2007, 261 (01) :68-73
[6]   Determination of phosphorus-, copper-, and zinc-containing human brain proteins by LA-ICPMS and MALDI-FTICR-MS [J].
Becker, JS ;
Zoriy, M ;
Becker, JS ;
Pickhardt, C ;
Damoc, E ;
Juhacz, G ;
Palkovits, M ;
Przybylski, M .
ANALYTICAL CHEMISTRY, 2005, 77 (18) :5851-5860
[7]   Imaging of copper, zinc, and other elements in thin section of human brain samples (Hippocampus) by laser ablation inductively coupled plasma mass spectrometry [J].
Becker, JS ;
Zoriy, MV ;
Pickhardt, C ;
Palomero-Gallagher, N ;
Zilles, K .
ANALYTICAL CHEMISTRY, 2005, 77 (10) :3208-3216
[8]   In-gel screening of phosphorus and copper, zinc and iron in proteins of yeast mitochondria by LA-ICP-MS and identification of phosphorylated protein structures by MALDI-FT-ICR-MS after separation with two-dimensional gel electrophoresis [J].
Becker, JS ;
Zoriy, M ;
Krause-Buchholz, U ;
Becker, JS ;
Pickhardt, C ;
Przybylski, M ;
Pompe, W ;
Rödel, G .
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, 2004, 19 (09) :1236-1243
[9]   Copper, zinc, phosphorus and sulfur distribution in thin section of rat brain tissues measured by laser ablation inductively coupled plasma mass spectrometry: possibility for small-size tumor analysis [J].
Becker, JS ;
Zoriy, MV ;
Pickhardta, C ;
Zilles, K .
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, 2005, 20 (09) :912-917
[10]   Applications of inductively coupled plasma mass spectrometry and laser ablation inductively coupled plasma mass spectrometry in materials science [J].
Becker, JS .
SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY, 2002, 57 (12) :1805-1820