A Suite of Solid-State NMR Experiments for RNA Intranucleotide Resonance Assignment in a 21 kDa Protein-RNA Complex

被引：27

作者：

Marchanka, Alexander ^{[1
]}

Simon, Bernd ^{[1
]}

Carlomagno, Teresa ^{[1
]}

机构：

[1] European Mol Biol Lab, Struct & Computat Biol Unit, D-69117 Heidelberg, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 38期

关键词：

NMR spectroscopy; RNA; RNA-protein complexes; structure elucidation; ANGLE-SPINNING NMR; BETA-AMYLOID FIBRILS; HIGH-RESOLUTION; RIBONUCLEOPROTEIN PARTICLE; CORRELATION SPECTROSCOPY; EXPERIMENTAL CONSTRAINTS; CONFORMATIONAL-ANALYSIS; CROSS-POLARIZATION; SIGNAL ASSIGNMENTS; MEMBRANE-PROTEINS;

D O I：

10.1002/anie.201304779

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Intranucleotide resonance of the 26mer box C/D RNA in complex with the L7Ae protein were assigned by solid-state NMR (ssNMR; see picture) spectroscopy. This investigation opens the way for studying RNA in large protein-RNA complexes by ssNMR spectroscopy. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：9996 / 10001

页数：6

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