The effects of non-thermal atmospheric pressure plasma jet on attachment of osteoblast

Despite the high success rate of dental implant surgery, the failures are still being reported and investigation have been undergone to improve attachment of osteoblast on the surface of implant material. With increasing interest in non-thermal atmospheric pressure plasma jet (NTAPPJ), the effects of it on the cellular mechanisms have been previously reported. Hence in this experiment, effects of NTAPPJ on osteoblast for improved attachment and possible application in dental implant surgery were investigated. Mouse osteoblast cells of MC3T3-E1 were first directly treated with NTAPPJ with air for various durations. Also to investigate the effects by culture media, culture media were separately treated with NTAPPJ for the same durations. Cell attachments were then assessed following 4 and 24 h of cell culture using Water Soluble Tetrazolium salt (WST) assay and confirmed by automated cell counter and examining under confocal laser microscope. The results showed that there was significantly improved osteoblast attachment with relatively short duration of NTAPPJ treatment. Also results indicated that NTAPPJ possibly improved osteoblast attachment through interactions with proteins in culture media that in turn interacted with cells. Hence the application of NTAPPJ on osteoblast improves cellular attachment and would be useful tool for dental implant surgery. (c) 2013 Elsevier B.V. All rights reserved.