Melatonin inhibits cell growth and migration, but promotes apoptosis in gastric cancer cell line, SGC7901

被引：39
作者：
Zhang, S. [1 ,2 ,3 ,4 ]
Qi, Y. [5 ]
Zhang, H. [3 ,4 ]
He, W. [3 ,4 ]
Zhou, Q. [3 ,4 ]
Gui, S. [6 ]
Wang, Y. [1 ,2 ,3 ,4 ]
机构：
[1] Anhui Med Univ, Mol Biol Lab, Hefei 230032, Anhui, Peoples R China
[2] Anhui Med Univ, Dept Biochem, Hefei 230032, Anhui, Peoples R China
[3] Anhui Med Univ, Key Lab Antiinflammatory & Immunol Pharmacol, Minist Educ, Hefei 230032, Anhui, Peoples R China
[4] Anhui Med Univ, Key Lab Gene Resource Utilizat Severe Dis Anhui P, Hefei 230032, Anhui, Peoples R China
[5] Second People Hosp Hefei, Gen Dept Hyperbar Oxygen, Hefei 230011, Peoples R China
[6] Anhui Med Univ, Dept Resp Dis, Hefei 230032, Anhui, Peoples R China
来源：
BIOTECHNIC & HISTOCHEMISTRY | 2013年 / 88卷 / 06期
基金：
中国国家自然科学基金;
关键词：
apoptosis; gastric cancer; melatonin; migration; SGC7901; LOW-DOSE INTERLEUKIN-2; PROLIFERATION; THERAPY; PLUS;
D O I：
10.3109/10520295.2013.769633
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The pineal hormone, melatonin (MLT), has been shown to have therapeutic effects in patients with gastric cancer; however, the mechanisms for the anti-cancer effects are unknown. We investigated the effects of melatonin on cell proliferation, apoptosis, colony formation and cell migration in the gastric adenocarcinoma cell line, SGC7901, using MTT assay, Hoechst 33258 staining, flow cytometry, western blot, caspase-3 activity assay, soft agar colony formation assay, and scratch-wound assay. Our results showed that melatonin could inhibit cell proliferation, colony formation and migration efficiency, and it promoted apoptosis of SGC7901 cells. Our findings suggest that the anti-cancer effects of melatonin may be due to both inhibition of tumor cell proliferation and reduction of the metastatic potential of tumor cells.
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收藏
页码：281 / 289
页数：9
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