Biological and Antibacterial Activities of the Natural Herb Houttuynia cordata Water Extract against the Intracellular Bacterial Pathogen Salmonella within the RAW 264.7 Macrophage

被引：57

作者：

Kim, Gon Sup ^{[1
]}

Kim, Dong Hyeok ^{[1
]}

Lim, Jeong Ju ^{[1
]}

Lee, Jin Ju ^{[1
]}

Han, Dae Yong ^{[1
]}

Lee, Whi Min ^{[3
]}

Jung, Won Chul ^{[1
]}

Min, Won Gi ^{[1
]}

Won, Chung Gil ^{[1
]}

Rhee, Man Hee ^{[3
]}

Lee, Hu Jang ^{[1
]}

Kim, Suk ^{[1
,2
]}

机构：

[1] Gyeongsang Natl Univ, Coll Vet Med, Inst Anim Med, Jinju 660701, South Korea

[2] Gyeongsang Natl Univ, Inst Agr & Life Sci, Jinju 660701, South Korea

[3] Kyungpook Natl Univ, Coll Vet Med, Dept Vet Med, Taegu 702701, South Korea

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2008年 / 31卷 / 11期

关键词：

salmonellosis; Houttuynia cordata; antibacterial activity; macrophage; Salmonella typhimurium; nitric oxide;

D O I：

10.1248/bpb.31.2012

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Salmonellosis is a major bacterial zoonosis that causes a variety of disease syndromes, from self-limiting enteritis to fatal infection in animals and food-borne infection and typhoid fever in humans. Recently, the emergence of multidrug-resistant strains of Salmonella sp. has caused more serious problems in public health. The present study investigated the antibacterial effects of Houttuynia cordata water extract (HCWE) against murine salmonellosis. In RAW 264.7 cells, there was no detectable cytotoxic effect of HCWE at any concentration between 25 and 100 mu g/ml after 8-h incubation. The antibacterial activity of HCWE was then examined in a Salmonella enterica serovar (Salmonella typhimurium), and was found to increase in a dose-dependent manner at concentrations from 25 to 100 mu g/ml during 8-h incubation. HCWE also affected RAW 264.7 cells including morphologic change and bacterial uptake, but there was no significant difference in bacterial replication in RAW 264.7 cells. With HCWE alone, nitric oxide (NO) production by RAW 264.7 cells did not increase, but when RAW 264.7 cells were infected by S. typhimurium, with or without HCWE, NO production with HCWE was 2-fold higher than that without HCWE. Treatment with HCWE did not affect inducible NO synthase (iNOS) mRNA expression by RAW 264.7 cells, but when RAW 264.7 cells with HCWE were infected by S. typhimurium, iNOS mRNA expression was increased during 8-h incubation. Furthermore, HCWE showed virulence reduction effects in S. typhimurium-infected BALB/c mice. After a lethal dose of S. typhimurium, the mortality rate in the HCWE untreated group was 100% at 7 d, but the HCWE 25, 50, and 100 mu g/ml groups survived until 11, 17, and 23 d, respectively. These data suggest that HCWE is stable and beneficial in the treatment of bacterial infection including intracellularly replicating pathogens and may solve antimicrobial misuse and overuse.

引用

页码：2012 / 2017

页数：6

共 43 条

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