DETECTION OF MYCOPLASMA PNEUMONIAE BY COLORIMETRIC LOOP-MEDIATED ISOTHERMAL AMPLIFICATION

被引：9

作者：

Zhao, Fei ^{[1
]}

Liu, Zhong ^{[2
]}

Gu, Yixin ^{[1
]}

Yang, Yuelian ^{[2
]}

Xiao, Di ^{[1
]}

Tao, Xiaoxia ^{[1
]}

Meng, Fanliang ^{[1
]}

He, Lihua ^{[1
]}

Zhang, Jianzhong ^{[1
]}

机构：

[1] Chinese Ctr Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Natl Inst Communicable Dis Control & Prevent, Beijing 102206, Peoples R China

[2] JiNan Municipal Ctr Dis Control & Prevent, Jinan 250021, Shandong, Peoples R China

来源：

ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA | 2013年 / 60卷 / 01期

关键词：

Mycoplasma pneumoniae; LAMP; clinical specimen; RAPID DETECTION; DIAGNOSIS; ASSAY;

D O I：

10.1556/AMicr.60.2013.1.1

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Mycoplasma pneumoniae (M. pneumoniae) is one of the most important pathogens that cause respiratory tract infection in children and adults. In this study, we describe a rapid and sensitive colorimetric loop mediated isothermal amplification (LAMP) method to detect M. pneumoniae. The specificity and sensitivity of this assay were detected with 21 common respiratory pathogens and 39 M. pneumoniae DNA. The sensitivity of LAMP was 100% among 39 M. pneumoniae isolates and the specificity was 100% among 9 members of other Mycoplasma and 12 common respiratory pathogens. The lowest detectable limit (LDL) of this assay was 10 2 copies, which detected by a series of standard M. pneumoniae DNA. To evaluate the clinical applicability of the LAMP assay, a total of 80 clinical samples were examined by conventional PCR, real-time PCR and the LAMP assays, respectively. The positive rates were 15.0%, 32.5% and 26.3%, respectively. This colorimetric LAMP assay demonstrated a high level of sensitivity comparable with that of conventional PCR for the detection of M. pneumoniae. It is a valuable method for simple, cost-effective and rapid detection of M. pneumoniae in the rural areas and basic clinical of China.

引用

页码：1 / 9

页数：9

共 19 条

[1] Evaluation of 12 commercial tests and the complement fixation test for Mycoplasma pneumoniae-specific immunoglobulin G (IgG) and IgM antibodies, with PCR used as the "gold standard" [J].