Comparison of hardwood and softwood dust-induced expression of cytokines and chemokines in mouse macrophage RAW 264.7 cells

被引:28
作者
Määttä, J
Luukkonen, R
Husgafvel-Pursiainen, K
Alenius, H
Savolainen, K
机构
[1] Finnish Inst Occupat Hlth, Dept Ind Hyg & Toxicol, Helsinki 00250, Finland
[2] Finnish Inst Occupat Hlth, Dept Epidemiol & Biostat, Helsinki 00250, Finland
关键词
wood dust; macrophage; inflammation; cytokine; chemokine; gene-expression;
D O I
10.1016/j.tox.2005.09.001
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Numerous epidemiological studies have shown that occupational exposure to wood dust can induce several respiratory diseases such as allergic rhinitis, chronic bronchitis, asthma, and sino-nasal adenocarcinoma. However, comparison of the harmful potential of different wood dust species on the basis of epidemiological studies is complicated because in the occupational environment workers are usually exposed to several wood dust species simultaneously. In the present study, we have characterized and compared the effects of two hardwood dusts, beech and teak, and two softwood dusts, pine and spruce, on cytokine and chemokine expression utilizing an in vitro model, murine macrophage cell line RAW 264.7. The expression patterns of selected cytokines and chemokines were assessed by real-time quantitative PCR and by ELISA. All the tested hardwood and softwood dusts induced TNF-alpha expression and inhibited IL-1 beta expression. Similarly, all the wood dusts induced the expression of CCL2, CCL3, CCL4, and CXCL2/3 chemokines and inhibited CCL24 expression. Our results indicate that both hardwood and softwood dusts influence the cytokine and chemokine expression of RAW 264.7 cells. Although some differences could be detected in the magnitude of responses to different wood dust species, the two tested wood dust groups, hardwoods and softwoods, have quite similar effects on cytokine and chemokine expression in RAW 264.7 cell line. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:13 / 21
页数:9
相关论文
共 36 条
[21]  
LI DH, 1990, AM J IND MED, V17, P84, DOI 10.1002/ajim.4700170123
[22]   ROS-mediated TNF-α and MIP-2 gene expression in alveolar macrophages exposed to pine dust [J].
Long, Huayan ;
Shi, Tingming ;
Borm, Paul J. ;
Maatta, Juha ;
Husgafvel-Pursiainen, Kirsti ;
Savolainen, Kai ;
Krombach, Fritz .
PARTICLE AND FIBRE TOXICOLOGY, 2004, 1 (01)
[23]  
MAATTA J, 2005, IN PRESS TOXICOLOGY
[24]   OCCUPATIONAL ASTHMA CAUSED BY OAK WOOD DUST [J].
MALO, JL ;
CARTIER, A ;
DESJARDINS, A ;
WEYER, RV ;
VANDENPLAS, O .
CHEST, 1995, 108 (03) :856-858
[25]  
Mandryk J, 1999, AM J IND MED, V35, P481, DOI 10.1002/(SICI)1097-0274(199905)35:5<481::AID-AJIM5>3.0.CO
[26]  
2-N
[27]   Interactions of fungi with phagocytes [J].
Mansour, MK ;
Levitz, SM .
CURRENT OPINION IN MICROBIOLOGY, 2002, 5 (04) :359-365
[28]   Takes your breath away - the immunology of allergic alveolitis [J].
McSharry, C ;
Anderson, K ;
Bourket, SJ ;
Boyd, G .
CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 2002, 128 (01) :3-9
[29]   The effects of IL-5 on airway physiology and inflammation in rats [J].
Nag, SS ;
Xu, LJ ;
Hamid, Q ;
Renzi, PM .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2003, 111 (03) :558-566
[30]   CARCINOGENIC EFFECTS OF WOOD DUST - REVIEW AND DISCUSSION [J].
NYLANDER, LA ;
DEMENT, JM .
AMERICAN JOURNAL OF INDUSTRIAL MEDICINE, 1993, 24 (05) :619-647