Proteomics of DF-1 cells infected with avian leukosis virus subgroup J

被引：13

作者：

Fan, Zhongjun ^{[2
]}

Hu, Xuming ^{[2
]}

Zhang, Yongpan ^{[2
]}

Yu, Chuan ^{[2
]}

Qian, Kun ^{[2
]}

Qin, Aijian ^{[1
,2
]}

机构：

[1] Yangzhou Univ, Coll Vet Med, Minist Educ, Key Lab Avian Prevent Med, Yangzhou 225009, Jiangsu, Peoples R China

[2] Yangzhou Univ, Coll Vet Med, Key Lab Jiangsu Prevent Vet Med, Yangzhou 225009, Jiangsu, Peoples R China

来源：

VIRUS RESEARCH | 2012年 / 167卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Avian leukosis virus; DF-1; cell; 2-DE; Proteomics; Pathogenesis; ACID-BINDING PROTEINS; METHIONINE AMINOPEPTIDASE-2; CELLULAR-PROTEINS; EXPRESSION; CANCER; IDENTIFICATION; APOPTOSIS; LAMIN; DJ-1; CYTOSKELETON;

D O I：

10.1016/j.virusres.2012.05.016

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Avian leukosis virus subgroup J (ALV-J) is an avian oncogenic retrovirus that has led to severe economic losses in the poultry industry in China in recent years. The pathogenesis of virus infection and virus-host interactions are still not well elucidated. In this paper, we investigated the expression changes for cellular proteins in DF-1 cells infected with ALV-J. Comparative analyses revealed that the majority of the altered proteins in DF-1 cells appeared at 6-12 h after ALV-J infection. Mass spectrometry identified 74 altered cellular proteins, including 30 up-regulated proteins and 44 down-regulated proteins. Some of these proteins are involved in cell cytoskeleton, metabolic processes, response to stimulus and immune responses. Other proteins, such as DJ-1, UCHL1, VDAC1 and HMGB1, have some relationship to apoptosis or oncogenesis. The changes in the transcriptional profile of DJ-1, UCHL1, VDAC1 and HMGB1 in infected as compared to uninfected DF-1 cells were confirmed by real-time RT-PCR. Our work gives some information about differential protein expression in cells infected with ALV-J, which will help us to understand viral pathogenicity. (c) 2012 Elsevier B.V. All rights reserved.

引用

页码：314 / 321

页数：8

共 60 条

[1] Proteomic analysis of cells in the early stages of herpes simplex virus type-1 infection reveals widespread changes in the host cell proteome [J].

Antrobus, Robin ;

Grant, Kyle ;

Gangadharan, Bevin ;

Chittenden, David ;

Everett, Roger D. ;

Zitzmann, Nicole ;

Boutell, Chris .

PROTEOMICS, 2009, 9 (15) :3913-3927

[2] Loss of lamin A/C expression in stage II and III colon cancer is associated with disease recurrence [J].

Belt, E. J. Th. ;

Fijneman, R. J. A. ;

van den Berg, E. G. ;

Bril, H. ;

Delis-van Diemen, P. M. ;

Tijssen, M. ;

van Essen, H. F. ;

de lange-de Klerk, E. S. M. ;

Belien, J. A. M. ;

Stockmann, H. B. A. C. ;

Meijer, S. ;

Meijer, G. A. .

EUROPEAN JOURNAL OF CANCER, 2011, 47 (12) :1837-1845

[3] Inhibition of HIV-1 replication in lymphocytes by mutants of the Rev cofactor eIF-5A [J].

Bevec, D ;

Jaksche, H ;

Oft, M ;

Wohl, T ;

Himmelspach, M ;

Pacher, A ;

Schebesta, M ;

Koettnitz, K ;

Dobrovnik, M ;

Csonga, R ;

Lottspeich, F ;

Hauber, J .

SCIENCE, 1996, 271 (5257) :1858-1860

[4]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[5] Blue silver: A very sensitive colloidal Coomassie G-250 staining for proteome analysis [J].

Candiano, G ;

Bruschi, M ;

Musante, L ;

Santucci, L ;

Ghiggeri, GM ;

Carnemolla, B ;

Orecchia, P ;

Zardi, L ;

Righetti, PG .

ELECTROPHORESIS, 2004, 25 (09) :1327-1333

[6] PTEN and the PI3-Kinase Pathway in Cancer [J].

Chalhoub, Nader ;

Baker, Suzanne J. .

ANNUAL REVIEW OF PATHOLOGY-MECHANISMS OF DISEASE, 2009, 4 :127-150

[7]

Cheng Zi-qiang, 2005, Weishengwu Xuebao, V45, P584

[8] Tumors Associated with Avian Leukosis Virus Subgroup J in Layer Hens during 2007 to 2009 in China [J].

Cheng, Ziqiang ;

Liu, Jianzhu ;

Cui, Zhizhong ;

Zhang, Li .

JOURNAL OF VETERINARY MEDICAL SCIENCE, 2010, 72 (08) :1027-1033

[9] Proteomic analysis of differentially expressed proteins in Penaeus vannamei hemocytes upon Taura syndrome virus infection [J].

Chongsatja, Phattara-orn ;

Bourchookarn, Apichai ;

Lo, Chu Fang ;

Thongboonkerd, Visith ;

Krittanai, Chartchai .

PROTEOMICS, 2007, 7 (19) :3592-3601

[10] The role of lamin A in cytoskeleton organization in colorectal cancer cells A proteomic investigation [J].

Foster, Clare R. ;

Robson, Joanne L. ;

Simon, William J. ;

Twigg, Jeremy ;

Cruikshank, Derek ;

Wilson, Robert G. ;

Hutchison, Christopher J. .

NUCLEUS, 2011, 2 (05) :434-443

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