Sensitive ECL sensor for sequence-specific DNA from Mycobacterium tuberculosis based on N-(aminobutyl)-N-ethylisoluminol functionalized gold nanoparticles labeling

被引：25

作者：

Yu, Xiuxia ^{[1
]}

Chai, Ying ^{[1
]}

Jiang, Jie ^{[1
]}

Cui, Hua ^{[1
]}

机构：

[1] Univ Sci & Technol China, Dept Chem, CAS Key Lab Soft Matter Chem, Hefei 230026, Anhui, Peoples R China

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY | 2012年 / 241卷

关键词：

Tuberculosis; Electrochemiluminescence; DNA sensor; N-(aminobutyl)-N-ethylisoluminol functionalized gold nanoparticles; ELECTROCHEMILUMINESCENCE IMMUNOSENSOR; NANOPROBE; AMPLIFICATION; IS6110;

D O I：

10.1016/j.jphotochem.2012.04.023

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A novel sensor was developed for highly sensitive detection of the sequence-specific DNA of Mycobacterium tuberculosis (MTB) by using N-(aminobutyl)-N-(ethylisoluminol) (ABEI) functionalized gold nanoparticles (ABEI-AuNPs) as labels and using 81 bp segment derived from the MTB specific insertion sequence IS6110 as the target strand. Owing to the novel ECL labels and amplification of gold nanoparticles and biotin-streptavidin system, extremely high sensitivity for ss-DNA could be achieved with a low detection limit of 3.3 x 10(-16) M and a wide linear dynamic range of 1.0 x 10(-15)-1.0 x 10(-12) M. The DNA sensor also showed good precision, good stability, low detection limit for the detection of sequence-specific DNA from MTB in serum samples, which might be of application potential in clinical diagnosis. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：45 / 51

页数：7

共 16 条

[11]

Tsuneyoshi T, 1997, RAPID COMMUN MASS SP, V11, P719, DOI 10.1002/(SICI)1097-0231(19970422)11:7<719::AID-RCM862>3.0.CO

[12]

2-J

[13] Visual detection of IS6110 of Mycobacterium tuberculosis in sputum samples using a test based on colloidal gold and latex beads [J].