BES/BZR Transcription Factor TaBZR2 Positively Regulates Drought Responses by Activation of TaGST1

被引:164
作者
Cui, Xiao-Yu [1 ,4 ]
Gao, Yuan [1 ]
Guo, Jun [2 ]
Yu, Tai-Fei [1 ]
Zheng, Wei-Jun [2 ]
Liu, Yong-Wei [3 ]
Chen, Jun [1 ]
Xu, Zhao-Shi [1 ]
Ma, You-Zhi [1 ]
机构
[1] Chinese Acad Agr Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Key Lab Biol & Genet Improvement Triticeae Crops, Inst Crop Sci,Minist Agr, Beijing 100081, Peoples R China
[2] Northwest A&F Univ, Coll Agron, Coll Plant Protect, Yangling 712100, Shaanxi, Peoples R China
[3] Hebei Acad Agr & Forestry Sci, Plant Genet Engn Ctr Hebei Prov, Inst Genet & Physiol, Shijiazhuang 050051, Hebei, Peoples R China
[4] Chinese Acad Agr Sci, Tobacco Res Inst, Qingdao 266101, Shandong, Peoples R China
基金
中国国家自然科学基金;
关键词
STRESS TOLERANCE; ARABIDOPSIS-THALIANA; GENE-EXPRESSION; ABSCISIC-ACID; CONFERS TOLERANCE; BRASSICA-NAPUS; BZR1; WHEAT; SALT; ETHYLENE;
D O I
10.1104/pp.19.00100
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
BRI1-EMS suppressor (BES)/brassinazole-resistant (BZR) family transcription factors are involved in a variety of physiological processes, but the biological functions of some BES/BZR transcription factors remain unknown; moreover, it is not clear if any of these proteins function in the regulation of plant stress responses. Here, wheat (Triticum aestivum) brassinazole-resistant 2 (TaBZR2)-overexpressing plants exhibited drought tolerant phenotypes, whereas downregulation of TaBZR2 in wheat by RNA interference resulted in elevated drought sensitivity. electrophoretic mobility shift assay and luciferase reporter analysis illustrate that TaBZR2 directly interacts with the gene promoter to activate the expression of T. aestivum glutathione s-transferase-1 (TaGST1), which functions positively in scavenging drought-induced superoxide anions (O-2(-) ). Moreover, TaBZR2 acts as a positive regulator in brassinosteroid (BR) signaling. Exogenous BR treatment enhanced TaBZR2-mediated O-2(-) - scavenging and antioxidant enzyme gene expression. Taken together, we demonstrate that a BES/BZR family transcription factor, TaBZR2, functions positively in drought responses by activating TaGST1 and mediates the crosstalk between BR and drought signaling pathways. Our results thus provide new insights into the mechanisms underlying how BES/BZR family transcription factors contribute to drought tolerance in wheat.
引用
收藏
页码:605 / 620
页数:16
相关论文
共 73 条
[1]   Differential expression analysis for sequence count data [J].
Anders, Simon ;
Huber, Wolfgang .
GENOME BIOLOGY, 2010, 11 (10)
[2]   Functions of OsBZR1 and 14-3-3 proteins in brassinosteroid signaling in rice [J].
Bai, Ming-Yi ;
Zhang, Li-Ying ;
Gampala, Srinivas S. ;
Zhu, Sheng-Wei ;
Song, Wen-Yuan ;
Chong, Kang ;
Wang, Zhi-Yong .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (34) :13839-13844
[3]  
Bechtold N, 1998, METH MOL B, V82, P259
[4]   Modulation of ethylene responses affects plant salt-stress responses [J].
Cao, Wan-Hong ;
Liu, Jun ;
He, Xin-Jian ;
Mu, Rui-Ling ;
Zhou, Hua-Lin ;
Chen, Shou-Yi ;
Zhang, Jin-Song .
PLANT PHYSIOLOGY, 2007, 143 (02) :707-719
[5]   Arabidopsis WRKY46, WRKY54, and WRKY70 Transcription Factors Are Involved in Brassinosteroid-Regulated Plant Growth and Drought Responses [J].
Chen, Jiani ;
Nolan, Trevor M. ;
Ye, Huaxun ;
Zhang, Mingcai ;
Tong, Hongning ;
Xin, Peiyong ;
Chu, Jinfang ;
Chu, Chengcai ;
Li, Zhaohu ;
Yin, Yanhai .
PLANT CELL, 2017, 29 (06) :1425-1439
[6]   Dual action of the active oxygen species during plant stress responses [J].
Dat, J ;
Vandenabeele, S ;
Vranová, E ;
Van Montagu, M ;
Inzé, D ;
Van Breusegem, F .
CELLULAR AND MOLECULAR LIFE SCIENCES, 2000, 57 (05) :779-795
[7]   Gene expression and functional analyses in brassinosteroid-mediated stress tolerance [J].
Divi, Uday K. ;
Rahman, Tawhidur ;
Krishna, Priti .
PLANT BIOTECHNOLOGY JOURNAL, 2016, 14 (01) :419-432
[8]   Brassinosteroid-mediated stress tolerance in Arabidopsis shows interactions with abscisic acid, ethylene and salicylic acid pathways [J].
Divi, Uday K. ;
Rahman, Tawhidur ;
Krishna, Priti .
BMC PLANT BIOLOGY, 2010, 10
[9]   Comprehensive comparison brassinosteroid-regulated of auxin-regulated and brassinosteroid-regulated genes in arabidopsis [J].
Goda, H ;
Sawa, S ;
Asami, T ;
Fujioka, S ;
Shimada, Y ;
Yoshida, S .
PLANT PHYSIOLOGY, 2004, 134 (04) :1555-1573
[10]   Soybean NAC transcription factors promote abiotic stress tolerance and lateral root formation in transgenic plants [J].
Hao, Yu-Jun ;
Wei, Wei ;
Song, Qing-Xin ;
Chen, Hao-Wei ;
Zhang, Yu-Qin ;
Wang, Fang ;
Zou, Hong-Feng ;
Lei, Gang ;
Tian, Ai-Guo ;
Zhang, Wan-Ke ;
Ma, Biao ;
Zhang, Jin-Song ;
Chen, Shou-Yi .
PLANT JOURNAL, 2011, 68 (02) :302-313