Laser and light-emitting diode effects on pre-osteoblast growth and differentiation

被引：49
作者：
Pagin, Marina Tochetti [1 ]
de Oliveira, Flavia Amadeu [2 ]
Oliveira, Rodrigo Cardoso [2 ]
Passanezi Sant'Ana, Adriana Campos [3 ]
Rubo de Rezende, Maria Lucia [3 ]
Aguiar Greghi, Sebastio Luiz [3 ]
Damante, Carla Andreotti [3 ]
机构：
[1] Bauru Sch Dent, BR-17012901 Sao Paulo, Brazil
[2] Bauru Sch Dent, Dept Biol Sci, BR-17012901 Sao Paulo, Brazil
[3] Bauru Sch Dent, Discipline Periodont, BR-17012901 Sao Paulo, Brazil
来源：
LASERS IN MEDICAL SCIENCE | 2014年 / 29卷 / 01期
关键词：
Lasers; Wound healing; Semiconductor; Phototherapy; Bone regeneration; OSTEOGENIC DIFFERENTIATION; IRRADIATION; CELLS; PROLIFERATION; CULTURE; PHOSPHATASE;
D O I：
10.1007/s10103-012-1238-5
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
The acceleration of bone regeneration by low-intensity laser irradiation may hold potential benefits in clinical therapy in orthopedics and dentistry. The purpose of this study is to compare the effects of light-emitting diode (LED) and laser on pre-osteoblast MC3T3 proliferation and differentiation. Cells were irradiated with red, infrared, and LED (3 and 5 J/cm(2)). Lasers had a power density of 1 W/cm(2) and irradiation time of 2 and 5 s. LED had a power density of 60 mW/cm(2) and irradiation time of 50 and 83 s. Control group did not receive irradiation. Cell growth was assessed by a colorimetric test (MTT) (24, 48, 72, and 96 h), and cell differentiation was evaluated by alkaline phosphatase (ALP) quantification after growth in osteogenic medium (72 and 96 h and 7 and 14 days). At 24 h, the cell growth was enhanced 3.6 times by LED (5 J/cm(2)), 6.8 times by red laser (3 J/cm(2)), and 10.1 times by red laser (5 J/cm(2)) in relation to control group (p < 0.05). At the other periods, there was no influence of irradiation on cell growth (p > 0.05). The production of ALP was not influenced by irradiation at any period of time (p > 0.05). Low-intensity laser and LED have similar effects on stimulation of cell growth, but no effect on cell differentiation.
引用
收藏
页码：55 / 59
页数：5
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