Study of the effect of water activity and temperature on ochratoxin A production by Aspergillus carbonarius

被引:43
作者
Esteban, A. [1 ]
Abarca, M. L. [1 ]
Bragulat, M. R. [1 ]
Cabanes, F. J. [1 ]
机构
[1] Univ Autonoma Barcelona, Fac Vet, Dept Sanitat Anat Anim, Grp Micol Vet, Barcelona 08193, Spain
关键词
Aspergillus carbonarius; coffee; grapes; ochratoxin A; temperature; water activity; wine;
D O I
10.1016/j.fm.2005.12.006
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The effect of water activity (a(w)) (0.78-0.99) and temperature (15 and 30 degrees C) on growth and production of ochratoxin A (OTA) of six Aspergillus carbonarius strains was studied in two culture media: Czapek yeast autolysate (CYA) agar and yeast extract sucrose (YES) agar, during a period of 30 days. The strains were selected to include different sources and different reported abilities to produce OTA and were characterized by RAPD and ITS-5.8S rDNA sequencing. CYA showed to be better culture medium than YES for OTA production in the isolates tested. OTA concentration was higher at 15 degrees C than at 30 degrees C. At 30 degrees C, ranges for OTA production were more restrictive than those for growth. OTA was produced from 0.86, 0.90 or 0.94 a(w) depending on the strain. At 15 degrees C, growth and OTA production were detected only in the 0.94-0.99 aw range. The molecular study performed showed that five of the strains were conspecific and no correlation was found between molecular data and the OTA production level or origin. The remaining strain had never been able to produce OTA and will probably represent a new species in the Aspergillus section Nigri. Our results show that A. carbonarius is able to grow and produce OTA in a wide range of water activities at both high and low temperatures. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码:634 / 640
页数:7
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