Cytotoxic Properties of Damiana (Tunera diffusa) Extracts and Constituents and A Validated Quantitative UHPLC-DAD Assay

被引：13

作者：

Willer, Johanna ^{[1
]}

Joehrer, Karin ^{[2
]}

Greil, Richard ^{[2
,3
]}

Zidorn, Christian ^{[1
]}

Cicek, Serhat Sezai ^{[1
]}

机构：

[1] Univ Kiel, Dept Pharmaceut Biol, Gutenbergstr 76, D-24118 Kiel, Germany

[2] Tyrolean Canc Res Inst, Innrain 66, A-6020 Innsbruck, Austria

[3] Paracelsus Med Univ Salzburg, Canc Cluster Salzburg, Dept Internal Med 3, Oncol Ctr,SCRI,LIMCR, Mullner Hauptstr 48, A-5020 Salzburg, Austria

来源：

MOLECULES | 2019年 / 24卷 / 05期

关键词：

multiple myeloma; quality control; naringenin; flavonoids; traditional preparation; TURNERA-DIFFUSA; MEDICINAL-PLANTS; FLAVONOIDS; APIGENIN; APOPTOSIS; CELLS;

D O I：

10.3390/molecules24050855

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In our continuing search for new cytotoxic agents, we assayed extracts, fractions, and pure compounds from damiana (Turnera diffusa) against multiple myeloma (NCI-H929, U266, and MMIS) cell lines. After a first liquid-liquid solvent extraction, the ethyl acetate layer of an acetone (70%) crude extract was identified as the most active fraction. Further separation of the active fraction led to the isolation of naringenin (1), three apigenin coumaroyl glucosides 2-4, and five flavone aglycones 5-9. Naringenin (1) and apigenin 7-O-(4"-O-p-E-coumaroyl)-glucoside (4) showed significant cytotoxic effects against the tested myeloma cell lines. Additionally, we established a validated ultra-high performance liquid chromatography diode array detector (UHPLC-DAD) method for the quantification of the isolated components in the herb and in traditional preparations of T. diffusa.

引用

页数：15

共 37 条

[31] Apigenin, Chrysin, and Luteolin Selectively Inhibit Chymotrypsin-Like and Trypsin-Like Proteasome Catalytic Activities in Tumor Cells
Wu, Yi-Xin
Fang, Xin
[J]. PLANTA MEDICA, 2010, 76 (02) : 128 - 132
[32] The acai flavonoid velutin is a potent anti-inflammatory agent: blockade of LPS-mediated TNF-α and IL-6 production through inhibiting NF-κB activation and MAPK pathway
Xie, Chenghui
Kang, Jie
Li, Zhimin
Schauss, Alexander G.
Badger, Thomas M.
Nagarajan, Shanmugam
Wu, Tong
Wu, Xianli
[J]. JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2012, 23 (09) : 1184 - 1191
[33] Synthesis and antiproliferative activity of novel aminoalkylated flavones
Yan, Lili
Liu, Haoran
Wang, Qiuan
Wang, Gangqiang
[J]. CHEMISTRY OF HETEROCYCLIC COMPOUNDS, 2017, 53 (08) : 871 - 875
[34] DNA topoisomerase I inhibitors: Cytotoxic flavones from Lethedon tannaensis
Zahir, A
Jossang, A
Bodo, B
Provost, J
Cosson, JP
Sevenet, T
[J]. JOURNAL OF NATURAL PRODUCTS, 1996, 59 (07): : 701 - 703
[35] Phytochemical Investigation of Turnera diffusa
Zhao, Jianping
Pawar, Rahul S.
Ali, Zulfiqar
Khan, Ikhlas A.
[J]. JOURNAL OF NATURAL PRODUCTS, 2007, 70 (02): : 289 - 292
[36] Anti-aromatase activity of the constituents from damiana (Turnera diffusa)
Zhao, Jianping
Dasmahapatra, Asok K.
Khan, Shabana I.
Khan, Ikhlas A.
[J]. JOURNAL OF ETHNOPHARMACOLOGY, 2008, 120 (03) : 387 - 393
[37] Apigenin inhibits proliferation and induces apoptosis in human multiple myeloma cells through targeting the trinity of CK2, Cdc37 and Hsp90
Zhao, Ming
Ma, Jian
Zhu, Hai-Yan
Zhang, Xu-Hui
Du, Zhi-Yan
Xu, Yuan-Ji
Yu, Xiao-Dan
[J]. MOLECULAR CANCER, 2011, 10

← 1 2 3 4 →