Detection and Identification of a 16SrII Group Phytoplasma Causing Clover Little Leaf Disease in Iran

被引:4
作者
Hosseini, Somayeh [1 ]
Bahar, Masoud [1 ]
Zirak, Leila [1 ]
机构
[1] Isfahan Univ Technol, Coll Agr, Dept Plant Protect, Esfahan, Iran
关键词
white clover; little leaf; phytoplasma; 16SrII; Iran; MOLECULAR-IDENTIFICATION;
D O I
10.1111/jph.12057
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
White clover plants showing little leaf and leaf reddening symptoms were observed in Isfahan Province in central Iran. Restriction fragment length polymorphism analyses of nested PCR-amplified fragments from Iranian clover little leaf phytoplasma isolates and representative phytoplasmas from other phytoplasma groups using AluI, CfoI, KpnI and RsaI restriction enzymes indicated that the clover phytoplasma isolates are related to the peanut WB group. Sequence analyses of partial 16S rRNA fragments showed that Iranian clover little leaf phytoplasma has 99% similarity with soybean witches'-broom phytoplasma, a member of the peanut WB (16SrII) phytoplasma group. This is the first report of clover infection with a phytoplasma related to the 16SrII group.
引用
收藏
页码:295 / 297
页数:3
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