Calcium Sensor for Photoacoustic Imaging

被引：112

作者：

Roberts, Sheryl ^{[1
,3
,4
]}

Seeger, Markus ^{[2
]}

Jiang, Yuanyuan ^{[3
,4
]}

Mishra, Anurag ^{[3
,4
]}

Sigmund, Felix ^{[3
,4
]}

Stelzl, Anja ^{[3
,4
]}

Lauri, Antonella ^{[1
,3
,4
]}

Symvoulidis, Panagiotis ^{[1
,3
]}

Rolbieski, Hannes ^{[1
,3
]}

Preller, Matthias ^{[5
,6
]}

Dean-Ben, X. Luis ^{[3
]}

Razansky, Daniel ^{[3
]}

Orschmann, Tanja ^{[4
]}

Desbordes, Sabrina C. ^{[4
]}

Vetschera, Paul ^{[2
]}

Bach, Thorsten ^{[7
]}

Ntziachristos, Vasilis ^{[2
,3
]}

Westmeyer, Gil G. ^{[1
,3
,4
]}

机构：

[1] Tech Univ Munich, Dept Nucl Med, D-81675 Munich, Germany

[2] Tech Univ Munich, Biol Imaging, D-81675 Munich, Germany

[3] Helmholtz Zentrum Munchen, Inst Biol & Med Imaging, D-85764 Oberschleissheim, Germany

[4] Helmholtz Zentrum Munchen, Inst Dev Genet, D-85764 Oberschleissheim, Germany

[5] CSSB, D-22607 Hamburg, Germany

[6] Hannover Med Sch, Inst Biophys Chem, D-30625 Hannover, Germany

[7] Tech Univ Munich, Dept Chem, D-85747 Garching, Germany

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2018年 / 140卷 / 08期

基金：

欧洲研究理事会;

关键词：

ARSENAZO-III; INDICATORS; MICROSCOPY; CA-2;

D O I：

10.1021/jacs.7b03064

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We introduce a selective and cell-permeable calcium sensor for photoacoustics (CaSPA), a versatile imaging technique that allows for fast volumetric mapping of photoabsorbing molecules with deep tissue penetration. To optimize for Ca2+-dependent photoacoustic signal changes, we synthesized a selective metallochromic sensor with high extinction coefficient, low quantum yield, and high photobleaching resistance. Micromolar concentrations of Ca2+ lead to a robust blueshift of the absorbance of CaSPA, which translated into an accompanying decrease of the peak photoacoustic signal. The acetoxymethyl esterified sensor variant was readily taken up by cells without toxic effects and thus allowed us for the first time to perform live imaging of Ca2+ fluxes in genetically unmodified cells and heart organoids as well as in zebrafish larval brain via combined fluorescence and photoacoustic imaging.

引用

页码：2718 / 2721

页数：4

共 21 条

[1] PROPERTIES OF THE METALLOCHROMIC DYES ARSENAZO-III, ANTIPYRYLAZO-III AND AZO1 IN FROG SKELETAL-MUSCLE FIBERS AT REST [J].

BAYLOR, SM ;

HOLLINGWORTH, S ;

HUI, CS ;

QUINTAFERREIRA, ME .

JOURNAL OF PHYSIOLOGY-LONDON, 1986, 377 :89-141

[2] Calcium signalling: Dynamics, homeostasis and remodelling [J].

Berridge, MJ ;

Bootman, MD ;

Roderick, HL .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2003, 4 (07) :517-529

[3] Evaluation of Arsenazo III as a Contrast Agent for Photoacoustic Detection of Micromolar Calcium Transients [J].

Cooley, Erika J. ;

Kruizinga, Pieter ;

Branch, Darren W. ;

Emelianov, Stanislav .

REPORTERS, MARKERS, DYES, NANOPARTICLES, AND MOLECULAR PROBES FOR BIOMEDICAL APPLICATIONS II, 2010, 7576

[4] In vitro photoacoustic sensing of calcium dynamics with arsenazo III [J].

Dana, N. ;

Fowler, R. A. ;

Allen, A. ;

Zoldan, J. ;

Suggs, L. ;

Emelianov, S. .

LASER PHYSICS LETTERS, 2016, 13 (07)

[5] A SURVEY OF THE AVAILABLE COLORIMETRIC INDICATORS FOR CA-2+ AND MG-2+ IONS IN BIOLOGICAL EXPERIMENTS [J].

DURHAM, ACH ;

WALTON, JM .

CELL CALCIUM, 1983, 4 (01) :47-55

[6] Noninvasive real-time visualization of multiple cerebral hemodynamic parameters in whole mouse brains using five-dimensional optoacoustic tomography [J].

Gottschalk, Sven ;

Fehm, Thomas Felix ;

Dean-Ben, Xose Luis ;

Razansky, Daniel .

JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 2015, 35 (04) :531-535

[7]

GRYNKIEWICZ G, 1985, J BIOL CHEM, V260, P3440

[8] Intrinsic Properties of Larval Zebrafish Neurons in Ethanol [J].

Ikeda, Hiromi ;

Delargy, Alison H. ;

Yokogawa, Tohei ;

Urban, Jason M. ;

Burgess, Harold A. ;

Ono, Fumihito .

PLOS ONE, 2013, 8 (05)

[9] AMPA receptor inhibition by synaptically released zinc [J].

Kalappa, Bopanna I. ;

Anderson, Charles T. ;

Goldberg, Jacob M. ;

Lippard, Stephen J. ;

Tzounopoulos, Thanos .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2015, 112 (51) :15749-15754

[10] Photoacoustic Probes for Ratiometric Imaging of Copper(II) [J].

Li, Hao ;

Zhang, Pamela ;

Smaga, Lukas P. ;

Hoffman, Ryan A. ;

Chan, Jefferson .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2015, 137 (50) :15628-15631

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